UHPLC Analysis of Vitamin K₁ and Related Compound on Ascentis^® Express C18

column

Ascentis Express C18 15 cm x 4.6 mm I.D., 5 μm particles (50537-U)

mobile phase

[A] methanol; [B] 2-propanol; (90:10, A:B)

flow rate

1.0 mL/min

pressure

1035 psi (71 bar)

column temp.

35 °C

detector

UV, 250 nm

injection

2 μL

sample

Vitamin K1 and related, 50 μg/mL 90:10, methanol:2-propanol