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UHPLC/MS Analysis of Flakka (α-PVP) and α-Pyrrolidinobutiophenone in Synthetic Urine on Ascentis® Express HILIC 2.7 μm

UHPLC/MS Analysis of Flakka (α-PVP) and α-Pyrrolidinobutiophenone in Synthetic Urine on Ascentis® Express HILIC 2.7 μm application for UHPLC

Materials

related product

Product No.
Description
Pricing

53934-U

Ascentis® Express HILIC, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 5 cm × 2.1 mm

P-090

α-Pyrrolidinovalerophenone hydrochloride solution

1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®

CONDITIONS

column

Ascentis Express HILIC, 5 cm x 2.1 mm I.D., 2.7 μm particles (53934-U)

mobile phase

5 mM ammonium formate in 95:5, acetonitrile:water

flow rate

0.5 mL/min

pressure

3275 psi (226 bar)

column temp.

35 °C ambient

detector

MS ESI+, MRM, 218.12 > 90.98, 232.13 > 91.04 m/z

injection

2 μL

sample

as prepared

Description

Analysis Note

The simple extraction from urine and rapid separation of the street drug Flakka (α-PVP) and a related compound is shown here on an Ascentis Express HILIC column in HILIC mode. Highest grade UHPLC solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.

Other Notes

matrix: 100 μL of horse plasma spiked with 200 ng/mL of each analytediluant:  900 μL acetonitrilevortex: 30 seccentrifuge:  10,000 rpm for 1 minfilter:  0.45 μm (Z227544)

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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