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Method 547: HPLC Analysis of Glyphosate and Glyphosate Degradation Product (Aminomethyl)phosphonic Acid (AMPA) on SUPELCOSIL™ SAX1","title":"EPA Method 547: HPLC Analysis of Glyphosate and Glyphosate Degradation Product (Aminomethyl)phosphonic Acid (AMPA) on SUPELCOSIL™ SAX1","uuid":"0a1e0b4b-101d-4df8-8409-1879fa9cb226","src":"/deepweb/assets/sigmaaldrich/marketing/global/images/technical-documents/protocols/analytical-chemistry/small-molecule-hplc/g006395-large/g006395-large.jpg","link":"","caption":"","displayPopupTitle":false,":type":"cms-commons/components/content/image"},"table":{"anchorId":null,"enableSort":false,"verticalTable":false,"headline":"Conditions","title":null,"caption":null,"table":"\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n

Column	SUPELCOSIL^™ SAX1, 25 cm x 4.6 mm I.D., 5 µm particles (59138)
Column temp.	38 °C
Mobile phase	[A] 5 mM monobasic potassium phosphate (pH 1.9 with phosphoric acid); [B] methanol; (A:B, 96:4)
Flow rate	0.5 mL/min
Sample	100 ng/mL in water
injection	100 µL
Detector	Fluorescence, excitation 338 nm, emission 455 nm
Detector	Post column conditions for the oxidation solution (see EPA Method 547): flow rate 0.2 mL/min, temperature 38 °C, reactor volume 500 µL; (post column conditions for the derivatization solution (see EPA Method 547): flow rate 0.5 mL/min, ambient temperature, mixing device 500 µL mixing loop (0.5 mm))

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Analysis Note	EPA Method 547 outlines the analysis of glyphosate in drinking water by direct aqueous injection HPLC, post column derivatization, and fluorescence detection. Anion exchange columns can be used for this method and have been shown to produce excellent results. A system is described here using a silica-based anion-exchange HPLC column (SUPELCOSIL^™ SAX1) instead of a polymer-based column. Advantages of the silica-based column include compatibility with organic solvents and higher pressure stability. These, in turn, lead to increased column lifetime.
Categories	Analytical Chromatography, Food & Beverage Analysis, Beverage Testing, Drinking Water and Bottled Water
Featured Industry	Environmental
Legal Information	SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC
Suitability	Application for HPLC

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