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DMEM/nutrient Mixture F-12 Formulation

DMEM/F12 medium is 1:1 mixture of Dulbecco's Modified Eagle's Medium (DMEM) and Ham's F-12 Nutrient Mixture. It is serum-free medium that contained insulin, transferrin, epidermal growth factor, leutinizing hormone or follicle stimulating hormone, somatomedin, and growth hormone first reported by Mather and Sato. DMEM/F12 media can also include HEPES buffer at a final concentration of 15 mM to compensate for the loss of buffering capacity from eliminating serum.

Click on the product numbers at the top of each column.

Component D0547
g/L		 D2906
g/L		 D6421
[1x] g/L		 D6434
[1x] g/L		 D8062
[1x] g/L		 D8437
[1x] g/L		 D8900
g/L		 D9785
g/L
                 
Inorganic Salts
Ammonium Molybdate • 4H2O 0.00000618 — — — — — — —
 
Ammonium Metavandate 0.00000058 — — — — — — —
 
Calcium Chloride 0.1166 0.1545 0.1166 0.1545 0.1166 0.1545 0.1545 —
 
Cupric Sulfate • 5H2O 0.0000013 0.0000013 0.0000013 0.0000013 0.0000013 0.0000013 0.0000013 0.0000013
 
Ferric Nitrate • 9H2O 0.00005 0.00005 0.00005 0.00005 0.00005 0.00005 0.00005 0.00005
 
Ferrous Sulfate • 7H2O 0.000417 0.000417 0.000417 0.000417 0.000417 0.000417 0.000417 0.000417
 
Manganese Sulfate 0.000000151 — — — — — — —
 
Magnesium Chloride • 6H2O 0.0612 0.0612 0.0612 0.0612 0.0612 0.0612 0.0612 —
 
Magnesium Sulfate (anhydrous) 0.04884 0.04884 0.04884 0.04884 0.04884 0.04884 0.04884 —
 
Nickel Chloride 0.00000012 — — — — — — —
 
Potassium Chloride 0.3118 0.3118 0.3118 0.3118 0.3118 0.3118 0.3118 0.3118
 
Sodium Bicarbonate — — 1.2 — — 1.2
 — —
 
Sodium Chloride 6.996 6.996 6.996 6.996 6.996 6.996 6.996 6.996
 
Sodium Metasilicate • 9H2O 0.0000142 — — — — — — —
 
Sodium Selenite 0.00000519 — — — — — — —
 
Sodium Phosphate Dibasic (anhydrous) 0.07102 0.07102 0.07102 0.07102 0.07102 0.07102 0.07102 0.07102
 
Sodium Phosphate Monobasic (anhydrous) 0.0543 0.0543 0.0543 0.0543 0.0543 0.0543 0.0543 0.0543
 
Stannous Chloride • 2H2O 0.00000011 — — — — — — —
 
Zinc Sulfate • 7H2O 0.000432 0.000432 0.000432 0.000432 0.000432 0.000432 0.000432 0.000432
 
Amino Acids
L-Alanine 0.00445 0.00445 0.00445 0.00445 0.00445 0.00445 0.00445 0.00445
 
L-Arginine • HCl 0.1475 0.1475 0.1475 0.1475 0.1475 0.1475 0.1475 0.1475
 
L-Asparagine • H2O 0.0075 0.0075 0.0075 0.0075 0.0075 0.0075 0.0075 0.0075
 
L-Aspartic Acid 0.00665 0.00665 0.00665 0.00665 0.00665 0.00665 0.00665 0.00665
 
L-Cystine • 2HCl 0.03129 0.03129 0.03129 0.03129 0.03129 0.03129 0.03129 0.03129
 
L-Cysteine • HCl • H2O 0.01756 0.01756 0.01756 0.01756 0.01756 0.01756 0.01756 0.01756
 
L-Glutamic Acid 0.00735 0.00735 0.00735 0.00735 0.00735 0.00735 0.00735 0.00735
 
L-Glutamine 0.365 0.365 — — 0.365 0.365 0.365 —
 
Glycine 0.01875 0.01875 0.01875 0.01875 0.01875 0.01875 0.01875 0.01875
 
L-Histidine • HCl • H2O 0.03148 0.03148 0.03148 0.03148 0.03148 0.03148 0.03148 0.03148
 
L-Isoleucine 0.05447 0.05447 0.05447 0.05447 0.05447 0.05447 0.05447 0.05447
 
L-Leucine 0.05905 0.05905 0.05905 0.05905 0.05905 0.05905 0.05905 —
 
L-Lysine • HCl 0.09125 0.09125 0.09125 0.09125 0.09125 0.09125 0.09125 —
 
L-Methionine 0.01724 0.01724 0.01724 0.01724 0.01724 0.01724 0.01724 —
 
L-Phenylalanine 0.03548 0.03548 0.03548 0.03548 0.03548 0.03548 0.03458 0.03548
 
L-Proline 0.01725 0.01725 0.01725 0.01725 0.01725 0.01725 0.01725 0.01725
 
L-Serine 0.02625 0.02625 0.02625 0.02625 0.02625 0.02625 0.02625 0.02625
 
L-Threonine 0.05345 0.05345 0.05345 0.05345 0.05345 0.05345 0.05345 0.05345
 
L-Tryptophan 0.00902 0.00902 0.00902 0.00902 0.00902 0.00902 0.00902 0.00902
 
L-Tyrosine • 2Na • 2H2O 0.05579 0.05579 0.05579 0.05579 0.05579 0.05579 0.05579 0.05579
 
L-Valine 0.05285 0.05285 0.05285 0.05285 0.05285 0.05285 0.05285 0.05285
 
Vitamins
D-Biotin 0.0000035 0.0000035 0.0000035 0.0000035 0.0000035 0.0000035 0.0000035 0.0000035
 
Choline Chloride 0.00898 0.00898 0.00898 0.00898 0.00898 0.00898 0.00898 0.00898
 
Folic Acid 0.00265 0.00265 0.00265 0.00266 0.00266 0.00266 0.00266 0.00266
 
myo-Inositol 0.0126 0.0126 0.0126 0.0126 0.0126 0.0126 0.0126 0.0126
 
Niacinamide 0.00202 0.00202 0.00202 0.00202 0.00202 0.00202 0.00202 0.00202
 
D-Pantothenic Acid (hemicalcium) 0.00224 0.00224 0.00224 0.00224 0.00224 0.00224 0.00224 0.00224
 
Pyridoxal • HCl 0.002 0.002 — 0.002 0.002 0.002 0.002 0.002
 
Pyridoxine • HCl 0.002031 0.002031 0.002031 — — 0.000031 0.000031 0.000031
 
Riboflavin 0.000219 0.000219 0.000219 0.000219 0.000219 0.000219 0.000219 0.000219
 
Thiamine • HCl 0.00217 0.00217 0.00217 0.00217 0.00217 0.00217 0.00217 0.00217
 
Vitamin B12 0.00068 0.00068 0.00068 0.00068 0.00068 0.00068 0.00068 0.00068
 
Other
D-Glucose 3.15 3.15 3.15 3.15 3.15 3.15 3.15 3.15
 
HEPES — 3.5745 3.5745 3.5745 — 3.5745 3.5745 3.5745
 
Hypoxanthine 0.0021 0.00244 0.0021 0.00244 0.0021 0.00244 0.00244 0.0021
 
Linoleic Acid 0.000042 0.000042 0.000042 0.000042 0.000042 0.000042 0.000042 0.000042
 
Phenol Red • Na 0.00863 — 0.00863 — 0.00863 0.00863 0.00863 —
 
Putrescine • HCl 0.000081 0.000081 0.000081 0.000081 0.000081 0.000081 0.000081 0.000081
 
Pyruvic Acid • Na 0.11 0.055 0.055 0.055 0.055 0.055 0.055 0.055
 
DL-Thioctic Acid 0.000105 0.000105 0.000105 0.000105 0.000105 0.000105 0.000105 0.000105
 
Thymidine 0.000365 0.000365 0.000365 0.000365 0.000365 0.000365 0.000365 0.000365
 
Add
Sodium Bicarbonate 1.2 1.2 — — — — 1.2 1.2
 
Calcium Chloride • 2H2O — — — — — — — 0.1545
 
L-Glutamine — — 0.365 0.365 — — — 0.365
 
L-Leucine — — — — — — — 0.05905
 
L-Lysine • HCl — — — — — — — 0.09125
 
Magnesium Chloride • 6H2O — — — — — — — 0.0612
 
Magnesium Sulfate — — — — — — — 0.04884
 
L-Methionine — — — — — — — 0.0172
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