Peptides are manufactured using solid phase FMOC or BOC chemistry methodologies on a PEG-Polystyrene support resin. Upon synthesis completion, side chain protecting groups are removed and the peptides are simultaneously cleaved from the resin. The cleaved and deprotected peptide material is then precipitated, washed and dissolved in a buffer containing H2O/ACN/HOAC prior to lyophilization.
Peptide QC is determined using ESI mass spectrometry (confirming full length product) and reverse phase HPLC (RP-HPLC; determining the peptide purity. Peptides not meeting required purity specifications are purified by RP-HPLC.
Figure 1.Solid Phase FMOC
Peptide stability is dependent on amino acid composition and sequence. Lyophilized peptide are generally more stable than those in solution. Storage of peptides in their lyophilized form at -20 oC or -80 oC will minimize degradation. Exposure to pH>8 and exposure to atmospheric oxygen should be minimized if possible. The following are potential degradation pathways for peptides:
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