DBS-FCL-2

Derived from 141 day old normal male African green monkey fetus. Cells are free of adventitious agents and are non-tumourigenic. Susceptible HSV, vaccinia, rhinovirus, mumps, rubella, parainfluenza 3, Coxsackie A9 and poliovirus types 1-3.

Monkey African Green lung

EMEM (EBSS) (M2279) + 2 mM L-Glutamine (G7513) + 1% NEAA (M7145) + 10% FBS / FCS (F2442).

Split sub-confluent cultures (70-80%) 1:2 to 1:3, i.e., seeding at 3-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37 °C. Finite lifespan, up to 12 population doublings are expected.

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.

This material is cited in a US and/or other Patent and may not be used to infringe parent claims. US Patent No. 4,040,905.