0.1 mM EDTA,
0.1 mM PMSF, and 25% glycerol.
Molecular mass: ∼63 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 63–85 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This
0.1 mM EDTA,
0.1 mM PMSF, and 25% glycerol.
Molecular mass: ∼63 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 63–85 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This
0.1 mM EDTA,
0.1 mM PMSF, and 25% glycerol.
Molecular mass: ∼63 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 63–85 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This
0.1 mM EDTA,
0.1 mM PMSF, and 25% glycerol.
Molecular mass: ∼63 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 63–85 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This
precipitation
procedures. The major phospholipids as assayed by
TLC are typically an average of 55%(42-63%)
phosphatidylcholine and 20%(10-32%)
phosphatidylethanolamine. Other trace components
such as
diluted in buffer
(B) to obtain standard solutions at the following
concentrations: 500, 250, 125, 63, 31 and
15 pg/0.1ml.
(B) Phosphate Buffered Saline, 0.01 M, pH 7.4, con-
taining 0.1% sodium azide
diluted in buffer (B) to obtain standard
solutions at the following concentrations:
500, 250, 125, 63, 31, 15, 8, and 4
pg/0.1 ml.
(B) Dilution buffer: 0.05 M Tris-HCl (Product
No. T3523), pH 8.0
calmodulin or
phosphodiesterase alone (Hansen, 1982). This antibody will recognize both the 61 and 63
kDa isoforms (CaM-PDE 1A and 1B, respectively) of active calmodulin-dependent
phosphodiesterase in
diluted in buffer (B) to obtain
standard solutions at the following concentrations:
500, 250, 125, 63, 31, and
15 pg/0.1 ml.
(B) Dilution buffer: 0.01 M phosphate buffered saline,
pH 7.4 containing
in normal human
serum to obtain standard solutions at the following
concentrations: 500, 250, 125, 63, and
31 pg/0.1ml.
(B) Phosphate Buffered Saline, 0.01M, pH 7.4, con-
taining 0.1% sodium azide.
diluted in buffer (B) to obtain standard
solutions at the following concentrations: 500, 250,
125, 63, 31, and 15 pg/0.1 ml.
B. Dilution buffer: 0.01 M phosphate buffered saline,
pH 7.4 containing
charcoal untreated powder 100-400 mesh
(Sigma Product No. C-5260), 0.1% Dextran
addition of charcoal. The dextran coated charcoal
suspension should be stirred and kept at 0(C in
ice-water for at least
further diluted in buffer (B) to
obtain standard solutions at the following
concentrations: 250, 125, 63, 31, 15, and 7.5
pg/0.1ml.
(B) Dilution buffer: 0.05M potassium phosphate buf-
fered saline, pH
diluted in buffer (B) to obtain standard solutions at
the following concentrations: 500, 250, 125, 63, 31,
and 15 pg/0.1 ml.
B. Dilution buffer: 0.01 M phosphate buffered saline,
pH 7.4 containing