performing nested PCR.
(1) The denaturation temperature can vary from +92°C to +95°C. +94°C is the standard denaturation temperature.
(2) Optimal annealing temperature depends on the melting temperature of
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We
Target siRNA NFS transfection
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2,908 µL of serum-containing medium and invert
several times to mix.
2. Carefully remove the culture medium from each
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Anti-STAT 1 (pTyr701) for 88-
92 kDa protein. It recognizes both human and mouse
STAT 1(pTyr701).
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