Determination
REAGENTS:
A. 90 mM Citrate Buffer Solution, pH 4.8 at 37°C
(Use Citrate Buffer Solution, Sigma Stock No. 104-4.)
B. 40 mM Tartrate Buffer, with 90 mM Citrate, pH 4.8 at 37°C.
(Use
Determination
REAGENTS:
A. 90 mM Citrate Buffer Solution, pH 4.8 at 37°C
(Use Citrate Buffer Solution, Sigma Stock No. 104-4.)
B. 40 mM Tartrate Buffer, with 90 mM Citrate, pH 4.8 at 37°C.
(Use
Determination
REAGENTS:
A. 90 mM Citrate Buffer Solution, pH 4.8 at 37°C
(Use Citrate Buffer Solution, Sigma Stock No. 104-4.)
B. 40 mM Tartrate Buffer, with 90 mM Citrate, pH 4.8 at 37°C.
(Use
Determination
REAGENTS:
A. 90 mM Citrate Buffer Solution, pH 4.8 at 37°C
(Use Citrate Buffer Solution, Sigma Stock No. 104-4.)
B. 40 mM Tartrate Buffer, with 90 mM Citrate, pH 4.8 at 37°C.
(Use
Agar 1.05465.0500 500 g 5 kg
MacConkey Agar - LI 30 ml 1.46022.0020 20 x 90 mm 120 x 90 mm
MacConkey Broth 1.05396.0500 500 g
Tryptic Soy Broth 1.05459.0500 500 g 5
Ambiant Temperature +0 °C to +40 °C (32 to 104 °F) +0 °C to +40 °C (32 to 104 °F) +0 °C to +40 °C (32 to 104 °F)
Environment Temperature 0 to 40 °C (32 to 104 ºF)
HCl.)
B. 15.2 mM p-Nitrophenyl Phosphate (PNPP)
(Prepare 5 ml in deionized water using Sigma 104 Phosphatase Substrate, Sigma Stock
No. 104-0.)
C. 100 mM Sodium Hydroxide Solution (NaOH)
(Prepare
HCl.)
B. 15.2 mM p-Nitrophenyl Phosphate (PNPP)
(Prepare 5 ml in deionized water using Sigma 104 Phosphatase Substrate, Sigma Stock
No. 104-0.)
C. 100 mM Sodium Hydroxide Solution (NaOH)
(Prepare
HCl.)
B. 15.2 mM p-Nitrophenyl Phosphate (PNPP)
(Prepare 5 ml in deionized water using Sigma 104 Phosphatase Substrate, Sigma Stock
No. 104-0.)
C. 100 mM Sodium Hydroxide Solution (NaOH)
(Prepare
HCl.)
B. 15.2 mM p-Nitrophenyl Phosphate (PNPP)
(Prepare 5 ml in deionized water using Sigma 104 Phosphatase Substrate, Sigma Stock
No. 104-0.)
C. 100 mM Sodium Hydroxide Solution (NaOH)
(Prepare
HCl.)
B. 15.2 mM p-Nitrophenyl Phosphate (PNPP)
(Prepare 5 ml in deionized water using Sigma 104 Phosphatase Substrate, Sigma Stock
No. 104-0.)
C. 100 mM Sodium Hydroxide Solution (NaOH)
(Prepare
HCl.)
B. 15.2 mM p-Nitrophenyl Phosphate (PNPP)
(Prepare 5 ml in deionized water using Sigma 104 Phosphatase Substrate, Sigma Stock
No. 104-0.)
C. 100 mM Sodium Hydroxide Solution (NaOH)
(Prepare
HCl.)
B. 15.2 mM p-Nitrophenyl Phosphate (PNPP)
(Prepare 5 ml in deionized water using Sigma 104 Phosphatase Substrate, Sigma Stock
No. 104-0.)
C. 100 mM Sodium Hydroxide Solution (NaOH)
(Prepare