1. Passage cells at 6 × 105 viable cells
per mL for either a 3-day passage or a
2-day passage. Do not allow cells to
reach viable cell density (VCD) greater
than 8 × 106 viable cells/mL for
optimal
(L/m2)
6
Filter performance examples.
Filter scalability test results for Millistak+® HC Pro
D0SP depth filter devices using a CHO feed stream
(mAb02, 20.5 x 106 TC/mL, 92% viable)
Human Myokine Magnetic Bead Panel
Catalog# HMYOMAG-56K
Control Catalog# HMY-6056, Lot# HMY-106 and HMY-206
Note: The Quality Control Ranges are generated with overnight assay format using serum
) of amino terminal inserts (N) of
29 amino acids each.
5
The six isoforms are
designated:
0N3R 0N4R
1N3R 1N4R
2N3R 2N4R
This product contains 6 recombinant Tau proteins
erythrocytes.
When assayed by flow cytometric analysis, using
10 µl of the antibody to stain 1 x 106 cells, a
fluorescence intensity is observed similar to that
obtained with saturating monoclonal
monocytes, thymocytes and T lymphocytes.
APPLICATIONS:
Flow cytometry: Use 10 µL per 106 cells
Immunohistochemistry: Frozen sections
Immunoprecipitation
Optimal working dilutions
exchange,
cell detachment
Media exchange~150 × 106 cells
~300−350
× 109 cells
Infection
180 L
180 L
600 g
80 L
120 g
40 L
20 g2 L
6 g
1× Mobius Bioreactor (3 L)
1× Mobius
Kahlert H., et al., Mol. Immunol., 29, 1191-201
(1992).
2. Externest D., et al., Infect Immun., 68, 3830-39
(2000).
3. Montelius C., et al., Br J Nutr., 106, 836-44 (2011).
4. Tober R., et al
al., J. Histochem. Cytochem., 29,
981-984 (1981).
10. Cyboron G.W. et al., J. Biol. Chem., 257, 4141-
4146 (1982).
11. Mayer, D. et al., Histochem. J., 23, 100-106 (1991).
12. Picher, M. et
Roles
of OsFKBP12 in Plant Defense System". The
Chinese University of Hong Kong, M.Phil. thesis,
p. 106 (2013).
11. Chong, Lisa Pui-Ling, “The role of ER-localized
HSP90 in modulating the WUSCHEL-CLAVATA
Performance
When assayed by flow cytometric analysis, using 5 µl of
the antibody to stain 1 x 106 cells, a fluorescence
intensity is observed similar to that obtained with
saturating monoclonal antibody
-100 x 106 cells,
depending on the cell type.
Note: Volumes below are for 50 -100 x 106 cells, harvested from one confluent 500 cm2 dish.
Remove medium from cells. Wash 50 -100 x 102. 6
incubate at 4 °C for 10 minutes.
5. Repeat steps 2-3.
6. Resuspend cells in initial volume of diluent.
7. For each sample, add 100 µl or 1 x 106 cells per
tube.
8. Add 10
Figure 6.
Figure 5. Stability of expression over passage.
The upper panel shows the percentage of cells expressing a mean peak current >500 pA at
40 mV at cell passages 9, 12, 19, 23, 29 and 35.