30 minutes at room temperature.
9. Repeat steps 2 and 3.
10. Resuspend cells in initial volume of diluent. For
each sample, add 100 µl or 1 x 106 cells per tube.
11. Add 10 µl of
metalloproteinases-1 (TIMP-1)
transcription. Biochem. Biophys. Acta, 1171, 41-55
(1992).
7. Gonzalez, E., et al., HIV-1 Tat inhibits IL-2 gene
transcription through qualitative and quantitative
alterations
Plasilova, M., et al., J. Med. Genet., 41,
609-614 (2004).
8. Musich, P.R. and Zou, Y., Aging, 1, 28-37 (2009).
9. Taimen, P., et al., Proc. Natl. Acad. Sci. USA, 106,
20788-20793 (2009).
chamber is then assembled using a
PVP-free polycarbonate filter (8 µm pore size) and
1 x 106 cells/well (2 day cultured human monocytes) is
added to the top chamber. After incubation for
75 minutes
quently, the entire cDNA contained in each
sample, corresponding to the templates of
approximately 2 x 106 peripheral blood
mononuclear cells (PBMCs) plus 1 x 104,
1 x 103, 1 x 102, 1 x 101, or 1 melanoma
. The Merck Index, 12th ed., Entry# 3308.
2. Lowe, O. G., Oxidation of thiols and disulfides to
sulfonic acids by dimethyl sulfoxide. J. Org.
Chem., 41(11), 2061 - 2064 (1976).
3. Advanced Organic
Total RNA was prepared from 100, 200, 300, 400,
500, and 600 µl of HeLa cell lysate (~ 1, 2, 3, 4, 5, & 6 x 106
cells) with either Sigma’s GenElute Mammalian Total RNA
Isolation Kit or with a RNA kit from
2H-pyran Dihydropyran (3,4-Dihydro-2-H-pyran)
®
See Reverse Side
Dihydropyran
(3,4-dihydro-2H-pyran)
May 1993
Technical Information Bulletin
Number AL-106
PRODUCT NO. D10,620-8
These
1994). Nature, 367:193.
59. Hoffman, Stanley, K.L. Crossin and G. M. Edelman, (1988). J. Cell. Biol. 106:519-532.
60. Chiquet-Ehrismann, R. (1990). FASEB J. 4:2598-2604.
61. Aukhil, I., et al, (1990). Matrix
1994). Nature, 367:193.
59. Hoffman, Stanley, K.L. Crossin and G. M. Edelman, (1988). J. Cell. Biol. 106:519-532.
60. Chiquet-Ehrismann, R. (1990). FASEB J. 4:2598-2604.
61. Aukhil, I., et al, (1990). Matrix
1994). Nature, 367:193.
59. Hoffman, Stanley, K.L. Crossin and G. M. Edelman, (1988). J. Cell. Biol. 106:519-532.
60. Chiquet-Ehrismann, R. (1990). FASEB J. 4:2598-2604.
61. Aukhil, I., et al, (1990). Matrix
Kunth. for potential
application as cosmeceuticals". Newcastle
University, Ph.D. dissertation, p. 106 (2019).
17. Roudnický, Pavel, "Molecular and Biochemical
Characterization and Localization of
slurry for several minutes.
– Wash cells in PBS* containing 0.5% Triton X-100.
Resuspend 1 to 2 × 106 cells in 100 μl of solution containing approximately 5 μg/ml Anti-Bromodeoxyuridine-
Fluorescein
senescence and should thus
be passaged every 3-4 days using a seeding density of 1-1.5×106 cells per T75
cm2 or 2-3×106 cells per T175 cm2 flask. Pre-washing with phosphate buffered
saline before harvesting
. The Merck Index, 12th ed., Entry# 3308.
2. Lowe, O. G., Oxidation of thiols and disulfides to
sulfonic acids by dimethyl sulfoxide. J. Org.
Chem., 41(11), 2061 - 2064 (1976).
3. Advanced Organic
hyperpolarization activated
cyclic nucleotide-gated potassium channel 2 (HCN2).
Format:
2 x 1 ml aliquots containing 1.14 x 106 cells/ml in 10% DMSO at passage 11.
Mycoplasma Testing:
The cell