September 2017
For 10 isolations of DNA from 100 to 1,000 mg mammalian tissue or 5 × 107 cultured cells
Cat. No. 11 814 770 001 1 kit
10 isolations
Store the kit at +2 to +8 °C.
Epichlorohydrin; 106-89-8] 및 이를 0.1% 이상 함유한 혼합물질
급성 흡입독성,
인화성 액체
2019-21 아크롤레인[Acrolein; 107-02-8] 및 이를 1.0% 이상 함유한 혼합물질
급성 흡입독성,
인화성 액체
2019-22 알릴 클로라이드[Allyl chloride ; 107-05-1] 및 이를 25%
above 1.5×106 cells/mL
– Freeze some cells (WCB 0%), with 1×107 viable cells/mL in Cellvento™ BHK-200 medium + 10% DMSO
– Continue with step 8.
8th adaptation of high density culture in Cellvento™
Total Bacteria Count Agar (TTC-Agar)
Bacteria
per ml 103 104 105 106 107
11-109-5_PB_100778:08-111-1_PB_113682 03.01.12 10:23 Seite 3
Status: December 2011
Merck KGaA, 64271 Darmstadt
Centrifuge the sample at 875 × g for 10 min (e.g., 2,000 rpm in a Sorvall RT6000B centrifuge).
– Discard the supernatant.
For 107 cells, centrifuge at 12,000 × g for 10 min following addition of glycogen
H4K5/8/12/16 displayed high efficiency for deacetylation as compared to the corresponding
non-acetylated peptides for all 3 enzymes tested.
0.0
2.0 x 107
0 5
4.0 x 107
6.0 x 107
8.0
Approximately 10-20 CV distilled water
For immediate re-use, equilibrate with buffer as
noted above.
For long-term storage, place the resin in 0.5 M NaCl
with bacteriostat and store at 2-8 ° C
Storage
ERalpha Hu-Cy5
SmartFlare™
RNA Detection Probe
Cat. # SF-107
pack size: 50µL (250 rxns)
Store at 2-8°C,
after reconstitution store at 23-27°C
DO NOT FREEZE
appropriate amount of cell lysis (RIPA)
buffer. Prepare 1 ml cell lysis buffer for the
extraction of 106−107 cells or 5−20 mg tissue.
Preparation of 1 ml cell lysis buffer
5X Buffer (Tris-EDTA)
Solution
10 mM DTNB
Solution
10 mM OAA Solution
Last to be added
1-3 CelLytic M Sample* x (2-8 µl) 186 – x µl 2 µl 2 µl 10 µl
4-6 Bicine Buffer Sample** x (2-8 µl) 186 –
φX–174 virus
particles at 50 SCFM per 10-inch cartridge.
Bacterial Aerosol Growth Retains B. diminuta liquid aerosol for 21 days at a minimum challenge of 107 CFU/cm2.
4
Specifications
% glutaraldehyde PBS per 2 x 107 cells. Invert the tube several
times to mix.
7. Incubate at room temperature for 10 minutes on an end-to end shaker or rotator.
8. Add 2 mL of