via G-tube
administration) within 15 min. Samples were collected at baseline
(fasting) and 5, 15, 30, 60, 90, 120, 150, 180, 210, and 240 min after
completion of the meal. All blood samples were collected
agglutinin or RCA may occur in
two forms designated RCA-60 or RCA-120, according
to their molecular weights of approximately 60 kDa
and 120 kDa, respectively. RCA-60 is composed of
an A- and B-chain. The
Separating
Resolution
5% 60–220 kDa
7.5% 30–120 kDa
10% 20–75 kDa
12% 17–65 kDa
15% 15–45 kDa
17.5% 12–30 kDa
D. Preparation of Gel Solutions
Prepare gel solutions, see Tables 4 and 5. These
tables give
Separating
Resolution
5% 60–220 kDa
7.5% 30–120 kDa
10% 20–75 kDa
12% 17–65 kDa
15% 15–45 kDa
17.5% 12–30 kDa
D. Preparation of Gel Solutions
Prepare gel solutions, see Tables 4 and 5. These
tables give
Separating
Resolution
5% 60–220 kDa
7.5% 30–120 kDa
10% 20–75 kDa
12% 17–65 kDa
15% 15–45 kDa
17.5% 12–30 kDa
D. Preparation of Gel Solutions
Prepare gel solutions, see Tables 4 and 5. These
tables give
total of 5 washings.
7. Tap the microwells (face down) on a layer of
absorbent paper to remove residual wash buffer.
8. Measure the required volume of TMB Substrate
(1 mL/strip or