Each tablet contains 30 mg of 4-Chloro-1-Naphthol
(4CN), a horseradish peroxidase substrate. It produces
an insoluble end product that is blue in color and can be
observed visually.1-3 The tablets (5, 50
50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25
mM DTT, 0.1 mM EGTA and 30% glycerol.
Purity: ≥ 75% (SDS-PAGE)
Molecular weight: ~142 kDa
Specific Activity: ≥ 10 units/mg protein (Bradford).
Please
acetone); may exist in
three polymorphic forms with melting point ranges of
149-152 °C, 146-148 °C and 142-144 °C.1
λmax: 260 nm (methanol, ethanol)1
Specific rotation = 0° (c = 1 in chloroform, 25 °C)
responds to the test for
Chloride conforms
Melting range between 140 °C and 145 °C 142–144 °C
Specific rotation, 50 mg per mL, in
water between –42° and –47° –45°
Loss on Drying
a working solution, dissolve the film
prepared in step 3 using a 4 mg/ml BSA solution
(fatty acid free Bovine Serum Albumin in water;
37 °C, 30 min. with repeated vortexing).
A working solution of 125
protein containing the calmodulin-binding domain
of myosin light chain kinase fused to amino acids 42-
142 of chicken Bcl-X as immunogen. This sequence
shares 75% homology with amino acids 46-146 of
human
detect 30 ng or less of human IgG by dot blot.
References
1. Beesley, J.E., Proc. Royal Micr. Soc., 20, 187
(1985).
2. Brada, D., and Roth, J., Anal. Biochem., 142, 79
(l984).
3.
.
Anti-Survivin (Prod. No. S 8191) is produced using a
peptide corresponding to amino acids 122-142 at the
carboxyl-terminus of human survivin. This sequence is
highly conserved (>70%) in rat and mouse
Anti-Interleukin-22 antibody produced in goat (I8658) - Enzyme Assay
L8658 Page 1 of 3
SPYEAS01
Revised: 07/30/96
SIGMA QUALITY CONTROL TEST
PROCEDURE
Enzymatic Assay of LYTICASE
PRINCIPLE:
containing 1% BSA and 0.1% NaN .3
2. FITC conjugated, isotype-matched, non-specific
rat or mouse immunoglobulin (Sigma Product No.
F-6522).
Procedure 3. After 30 minutes, add 2 ml of cold diluent
rat or mouse
immunoglobulin at the same concentration as
test antibody, followed by steps 3 - 8.
3. After 30 minutes, add 2 ml of cold diluent to all
tubes.
minutes.
5. Remove supernatant by careful
higher order chromosome
architecture without enzymatically modifying DNA.
J. Cell. Biochem. 69, 127-142, (1998).
8. Kopel, V., et al., Unwinding of the third strand of a
DNA triple helix, a novel activity
G., Proc. Natl. Acad. Sci.
USA, 75, 4001 (1978).
2. Massagne, J., Prog. Med. Virol., 32, 142 (1985).
3. Anzano, M., et al., Cancer Res., 42, 4776 (1982).
4. Anzano, M., et al., Proc. Natl. Acad.
higher order chromosome
architecture without enzymatically modifying DNA.
J. Cell. Biochem. 69, 127-142, (1998).
8. Kopel, V., et al., Unwinding of the third strand of a
DNA triple helix, a novel activity
.5:1
acrylamide:bisacrysmide) minigel (1.5 mm width) at 30 mA/gel ~1-1.5 hours.
3. Transfer in semi-dry system under standard conditions (3 h 100 mA for two minigel gels)
4. Stain the transferred