Batches/yr 110 109 152 152
Bottleneck
unit operation
Production Production Production Production
Bottleneck time
(days/batch)
2.39 2.40 1.73 1.73
Batches/yr 110 109 152 152
Total Cost of
Batches/yr 110 109 152 152
Bottleneck
unit operation
Production Production Production Production
Bottleneck time
(days/batch)
2.39 2.40 1.73 1.73
Batches/yr 110 109 152 152
Total Cost of
phenylchloroformates and modification of ribonuclease and superoxide
dismutase. Appl. Biochem. Biotechnol., 11, 141-152 (1985).
2. Martindale The Extra Pharmacopoeia, 30th ed., Reynolds, J. E. F., ed., The Pharmaceutical
et al., Prog. Clin. Biol. Res., 286B, 393
(1988)
10. Ozaki, H., et al., Eur. J. Biochem., 152, 475 (1985)
11. Farley, R. A., et al., J. Biol. Chem., 276, 2608
(2001).
12. Kuroki, D. W., et al. Cancer
and temperatures are as follows:4
Conditions (all in 0.1 M KPO4 buffer) Half life (Hours)
pH 6.5 at 20°C 40
pH 7.5 at 20°C 10
pH 8.5 at 20°C 1.4
pH 8.5 at 0°C 11
1992).
11. Catalona, W.J. et al., Selection of optimal prostate
specific antigen cut-offs for early detection of
prostate cancer: receiver operating characteristic
curves. J. Urol., 152, 2037-2042
and Saelinger, C.B., Preparation of
Pseudomonas exotoxin A. Methods in
Enzymology, 165, 147-152 (1988).
2. O'Brien, A.D., et al., Biological properties of
Shigella flexneri 2A toxin and its
phenylchloroformates and modification
of ribonuclease and superoxide dismutase. Appl.
Biochem. Biotechnol., 11, 141-152 (1985).
2. Ingham, K.C., Precipitation of Proteins with
Polyethylene Glycol. Meth. Enzymol
glycerol.
Molecular mass: ∼86 kDa
Purity: 70–95% (SDS-PAGE, see Figure 1)
Specific Activity: 112–152 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This product is for R&D use only, not for
of RNA. Improves
purity of the initial RNA precipitate and avoids excessive foaming Meth. Enzymol. 152, 219
(1987)
6. Wroblewski, W., Solubilization of Spiroplasma citri cell membrane proteins with
A8.16.
3. Wallace, D. M., Large- and Small-Scale Phenol
Extractions, Meth. Enzymol., 152, 33-41 (1987).
4. Baniecki, M. L., et al., Adenovirus proteinase:
crystallization and preliminary X-ray
A8.16.
3. Wallace, D. M., Large- and Small-Scale Phenol
Extractions, Meth. Enzymol., 152, 33-41 (1987).
4. Baniecki, M. L., et al., Adenovirus proteinase:
crystallization and preliminary X-ray
A8.16.
3. Wallace, D. M., Large- and Small-Scale Phenol
Extractions, Meth. Enzymol., 152, 33-41 (1987).
4. Baniecki, M. L., et al., Adenovirus proteinase:
crystallization and preliminary X-ray
Phenylchloroformates and Modification
of Ribonuclease and Superoxide Dismutase. Appl.
Biochem. Biotechnol., 11(2), 141-152 (1985).
2. Ingham, K. C., Precipitation of Proteins with
Polyethylene Glycol. Meth. Enzymol
Phenylchloroformates and Modification
of Ribonuclease and Superoxide Dismutase. Appl.
Biochem. Biotechnol., 11(2), 141-152 (1985).
2. Ingham, K. C., Precipitation of Proteins with
Polyethylene Glycol. Meth. Enzymol
phenylchloroformates and modification
of ribonuclease and superoxide dismutase. Appl.
Biochem. Biotechnol., 11, 141-152 (1985).
2. Ingham, K.C., Precipitation of Proteins with
Polyethylene Glycol. Meth. Enzymol
phenylchloroformates and modification
of ribonuclease and superoxide dismutase. Appl.
Biochem. Biotechnol., 11, 141-152 (1985).
2. Ingham, K.C., Precipitation of Proteins with
Polyethylene Glycol. Meth. Enzymol
Phenylchloroformat es and Modification
of Ribonuclease and Superoxide Dismutase. Appl.
Biochem. Biotechnol., 11(2), 141-152 (1985).
2. Ingham, K. C., Precipitation of Proteins with
Polyethylene Glycol. Meth. Enzymol
multidimensional approach to
marine lipid class and molecular species analysis.
J. Chromatogr. Sci., 39(4), 146-152 (2001).
2. Vercauteren, J., et al., Stir bar sorptive extraction
for the determination of ppq-level
(1) 148-152 (1995).
6. Slordal, L., et al., A sensitive and simple high-
performance liquid chromatographic method for
the determination of mitoxantrone in plasma. Ther.
Drug Monit., 15(4), 328-333
A8.16.
3. Wallace, D. M., Large- and Small-Scale Phenol
Extractions, Meth. Enzymol., 152, 33-41 (1987).
4. Baniecki, M. L., et al., Adenovirus proteinase:
crystallization and preliminary X-ray