Centrifuge cells at 200 x g for 5 min. Discard the supernatant and retain
the cell pellet.
5. Resuspend the cell pellet at 1 x 106 cells/ml in cold DiOC2(3) or
Rhodamine 123 Loading Buffer.
proliferation. Cell, 123:409-421.
Important Note: During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For
products with volumes of 200 µL or less
use freshly prepared
extracts.
Immunofluorescence: a working antibody dilution of
1:100-1:200 is recommended using mouse 3T3 and rat
NRK cells fixed and permeabilized with 4%
paraformaldehyde
Recommended Dilutions: Recommended starting dilution
for Western blot analysis is 1:500, for tissue or cell staining
1:200.
Note: Optimal concentrations and conditions for each
application should be determined
Recommended Dilutions: Recommended starting dilution
for Western blot analysis is 1:500, for tissue or cell staining
1:200.
Note: Optimal concentrations and conditions for each
application should be determined
Recommended Dilutions: Recommended starting dilution
for Western blot analysis is 1:500, for tissue or cell staining
1:200.
Note: Optimal concentrations and conditions for each
application should be determined
Recommended Dilutions: Recommended starting dilution
for Western blot analysis is 1:500, for tissue or cell staining
1:200.
Note: Optimal concentrations and conditions for each
application should be determined
-- Autoclave at 123 °C for 90 min 2.0
Non-Sterile Capsules
Millipak 100, MP-L1---- Autoclave at 123 °C for 90 min 2.5
Non-Sterile Capsules
Millipak 200, MP-L2---- Autoclave at 123 °C for 90 min 5.0
(1992) 1160:87-94.
REFERENCES: Brain Research (1996) 735:154-158.
Brain Research (1996) 741:123.
J. Neuroscience (1997) 17:4764-4784.
J. Neuroscience (1997) 17:8804-8816.
Brain Research (1999
differences in alternative splicing of the
NR1 protein in rat cortex and cerebellum, Dev. Brain Res. 123:143-150, 2000.
Al-Hallaq, R., Yasuda, R.P. and Wolfe, B.B.: Enrichment of NMDA NR1 splice variants
and 25%
glycerol.
Molecular mass: ∼85 kDa
Purity: 70–95% (SDS-PAGE, see Figure 1)
Specific Activity: 91–123 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This product is for R&D use
-3731; 4) Matsuda LA et al
(1990) Nature 346:561-564; 5) Ho BY et al (1996) Neurosci. Lett. 212:123-126; 6)
Chakraborty A et al (1995) DNA seq. 5:385-388.
Important Note: During shipment
mOsm/kg
Ionized Calcium (calc) 3.9 mg/dL
Total Iron 95 µg/dL
Cholesterol 165 mg/dL
Triglycerides 123 mg/dL
pH 7.2
Preservatives None
STORAGE/HANDLING: Store frozen at -20°C for up to 12 months after
following base pair lengths (1 base pair = 660 daltons).
Fragment lengths are derived from computer analysis of the pBR322 sequence.
bp
587 540 504 458 434 267 234 213 192 184 124 123 104 89 80 64 57
asymmetric synthesis. Part I. Asymmetric addition
of malonate nucleophiles. J. Am. Chem. Soc.,
123(16), 3671-3686 (2001).
4. Valdes, T. I., and Moussy, F., A ferric chloride pre-
treatment to prevent
concentrations of the antibody
for 1 hour at 37 °C in a 96 well plate. Following this
preincubation period, D10.G4.1 cells were added. The
assay mixture in a total volume of 200 µL, containing
antibody at
10. Sanz-Vicente, I. et al., Microchem. J., 123, 259-266
(2015).
Trizma is a registered trademark of Sigma-Aldrich Co.
LLC.
GCY,MAM 12/16-1
2016 Sigma-Aldrich Co. LLC. All rights
dilutions by titration assay.
References
1. Cassano, W., J. Immunol. Meth., 117, 169 (1989).
2. Wilchek, M., and Bayer, E., Methods in Enzymol.,
184, 123 (1990).
ExtrAvidin is a registered trademark
up to 6 months. Avoid
repeated freeze/thaw cycles.
REFERENCES:
1. Atkinson, L., et al., Neuroscience (2004) 123:761-768.
2. Liu, S., et al., J. Neuroscience (2003) 23:in press.
3. J. Neuroscience
differences in alternative splicing of the
NR1 protein in rat cortex and cerebellum, Dev. Brain Res. 123:143-150, 2000.
Al-Hallaq, R., Yasuda, R.P. and Wolfe, B.B.: Enrichment of NMDA NR1 splice variants
et al., Ann. Rev. Immunol., 3, 263
(1985).
4. Aarden, L., et al., J. Immunol., 123, 2928 (1979).
RC,SC,PHC 07/11-1
Sigma brand products are sold through Sigma-Aldrich, Inc.
Sigma-Aldrich, Inc.
, and 25% glycerol.
Molecular mass: ∼70 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 91–123 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This product is for R&D use
, and 25% glycerol.
Molecular mass: ∼70 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 91–123 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This product is for R&D use
, and 25% glycerol.
Molecular mass: ∼70 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 91–123 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This product is for R&D use