,
382(2), 232-237 (2000).
7. Lund, A. et al., Am. J. Physiol. Renal Physiol.,
284(6), F1226-F1234 (2003).
8. Wetter, T. et al., Med. Mycol., 43(6), 525-532
(2005).
9. Baye, T. et al.
including 10 control reactions 05 081 955 001
Synthesis Kit kit for 100 reactions 05 091 284 001
kit for 200 reactions 05 081 963 001
Please note that in the 2008 catalog, the catalog numbers for Transcriptor
, Casein kinase 2: an 'eminence grise' in
cellular regulation? Biochem. Biophys. Acta, 1054,
267-284 (1990).
4. Litchfield, D.W., and Luscher, B., Casein kinase II
in signal transduction and cell
mRNAs in the central nervous system: a hybridization histochemical study in the rat. J.
Comp. Neurol. 284:314-335.
Wada E, McKinnon D, Heinemann S, Patrick J, Swanson LW (1990) The distribution of
mRNA
, Casein kinase 2: an 'eminence grise' in
cellular regulation? Biochem. Biophys. Acta, 1054,
267-284 (1990).
3. Litchfield, D.W., and Luscher, B., Casein kinase II
in signal transduction and cell
Keppler, OT et al (1999) 'UDP-GlcNAc 2-epimerase: A regulator of cell
surface sialylation Science 284, 1372-1376.
1 A260 unit Concentration (mg/ml)
Single-stranded RNA 40
mRNA Isolation Kit y Version
certificate of
analysis.
References 1 Brown, N. L. & Smith, M. (1976) FEBS Lett. 65, 284.
2 Malyguine, E. et al. (1980) Gene 8, 163.
3 Kessler, C. & Manta,V. (1990) Gene 92, 1–248.
4 Rebase The Restriction
types and must be
determined empirically.
8. Change medium every day for sustaining expression of SimpliconTM RNA. Add fresh 10-20% of
B18R-CM or 200 ng/mL B18R protein and puromycin with each media
types and must be
determined empirically.
8. Change medium every day for sustaining expression of SimpliconTM RNA. Add fresh 10-20% of
B18R-CM or 200 ng/mL B18R protein and puromycin with each media
non-phospho-Tyr1173) 20G3 H M IP WB M IgG1κ 50 µg 05-484
Anti-Epidermal Growth Factor 144-8 H M IP IH SPur M IgG1 200 µL MAB126
Anti-EGF Receptor, intracellular, activated 74 H ELISA WB Pur M IgG1 100 µg
system.
REFERENCES
1. Stebbins, C. E., Kaelin, W. G. T. Jr.,
and Pavletich, N. P. (1999) Science 284,
455–461.
2. Shuman, S. (1990) J. Biol. Chem. 265,
11960–11966.
3. Li, C., Schwabe, J. W., Banayo
4. Pittenger, M. F., Mackay, A. M., Beck, S. C., Jaiswal, R. K.,
Douglas, R. (1999). Science 284: 143-147.
5. Salasznyk, R. M., Williams W. A., Boskey, A., Batorsky, A.,
and Plopper, G. E. (2004
NP_002476.2 y n/d y
490 Nedd 8 N2786 4738 NEDD8 M NP_006147.1 y n/d n/d
498 Neurofilament 160 N2787 4741 NEFM M NP_001099011.1 ,NP_005373.2 y y y
499 Neurofilament 200 N4142 4744 NEFH P NP_066554.2
NP_002476.2 y n/d y
490 Nedd 8 N2786 4738 NEDD8 M NP_006147.1 y n/d n/d
498 Neurofilament 160 N2787 4741 NEFM M NP_001099011.1 ,NP_005373.2 y y y
499 Neurofilament 200 N4142 4744 NEFH P NP_066554.2
NP_002476.2 y n/d y
490 Nedd 8 N2786 4738 NEDD8 M NP_006147.1 y n/d n/d
498 Neurofilament 160 N2787 4741 NEFM M NP_001099011.1 ,NP_005373.2 y y y
499 Neurofilament 200 N4142 4744 NEFH P NP_066554.2
M., Yamagami, T.,
Taira, T., Aso, Y., and Ishiguro, M.
(2002) Biosci. Biotechnol. Biochem.
66, 277–284.
13
PROTEIN DELIVERY INTO CELLS
17
Introduction of a target protein intocells permits functional
17. Pittenger, M.F., et al. Multilineage potential of adult human
mesenchymal stem cells. Science 284, 143, 1999.
276 RAUH ET AL.
18. Kassem, M., and Abdallah, B.M. Human bone-marrow-
derived