immediately,
evaporate samples under a stream of nitrogen.
9. Add 50 µL ethanol to the dried samples and
reconstitute sample with at least 200 µL of Assay
Buffer or cell culture media.
10. If analysis
K. et al., The muscle protein Dok-7 is
essential for neuromuscular synaptogenesis.
Science, 312, 1802-1805 (2006).
2. Kim C.H. et al., Regulation of MuSK expression by
a novel signaling pathway
K. et al., The muscle protein Dok-7 is
essential for neuromuscular synaptogenesis.
Science, 312, 1802-1805 (2006).
2. Kim C.H. et al., Regulation of MuSK expression by
a novel signaling pathway
K. et al., The muscle protein Dok-7 is
essential for neuromuscular synaptogenesis.
Science, 312, 1802-1805 (2006).
2. Kim C.H. et al., Regulation of MuSK expression by
a novel signaling pathway
K. et al., The muscle protein Dok-7 is
essential for neuromuscular synaptogenesis.
Science, 312, 1802-1805 (2006).
2. Kim C.H. et al., Regulation of MuSK expression by
a novel signaling pathway
K. et al., The muscle protein Dok-7 is
essential for neuromuscular synaptogenesis.
Science, 312, 1802-1805 (2006).
2. Kim C.H. et al., Regulation of MuSK expression by
a novel signaling pathway
K. et al., The muscle protein Dok-7 is
essential for neuromuscular synaptogenesis.
Science, 312, 1802-1805 (2006).
2. Kim C.H. et al., Regulation of MuSK expression by
a novel signaling pathway
cells can
be stained with AO (Section 9).
13. If cells are to be monitored using Hoechst stain (Section 8):
a. Add 1.6 µl Hoechst stain (0.5% v/v) to each 312 µl of cell overlay material
critical role of
protein kinase C-theta in Fas/Fas ligand-mediated
apoptosis. J. Immunol., 178, 312-319 (2007).
2. Kim, J K. et al., PKC-theta knockout mice are
protected from fat-induced insulin
critical role of
protein kinase C-theta in Fas/Fas ligand-mediated
apoptosis. J. Immunol., 178, 312-319 (2007).
2. Kim, J K. et al., PKC-theta knockout mice are
protected from fat-induced insulin
critical role of
protein kinase C-theta in Fas/Fas ligand-mediated
apoptosis. J. Immunol., 178, 312-319 (2007).
2. Kim, J K. et al., PKC-theta knockout mice are
protected from fat-induced insulin
critical role of
protein kinase C-theta in Fas/Fas ligand-mediated
apoptosis. J. Immunol., 178, 312-319 (2007).
2. Kim, J K. et al., PKC-theta knockout mice are
protected from fat-induced insulin
critical role of
protein kinase C-theta in Fas/Fas ligand-mediated
apoptosis. J. Immunol., 178, 312-319 (2007).
2. Kim, J K. et al., PKC-theta knockout mice are
protected from fat-induced insulin
critical role of
protein kinase C-theta in Fas/Fas ligand-mediated
apoptosis. J. Immunol., 178, 312-319 (2007).
2. Kim, J K. et al., PKC-theta knockout mice are
protected from fat-induced insulin
critical role of
protein kinase C-theta in Fas/Fas ligand-mediated
apoptosis. J. Immunol., 178, 312-319 (2007).
2. Kim, J K. et al., PKC-theta knockout mice are
protected from fat-induced insulin
critical role of
protein kinase C-theta in Fas/Fas ligand-mediated
apoptosis. J. Immunol., 178, 312-319 (2007).
2. Kim, J K. et al., PKC-theta knockout mice are
protected from fat-induced insulin
critical role of
protein kinase C-theta in Fas/Fas ligand-mediated
apoptosis. J. Immunol., 178, 312-319 (2007).
2. Kim, J K. et al., PKC-theta knockout mice are
protected from fat-induced insulin
critical role of
protein kinase C-theta in Fas/Fas ligand-mediated
apoptosis. J. Immunol., 178, 312-319 (2007).
2. Kim, J K. et al., PKC-theta knockout mice are
protected from fat-induced insulin
critical role of
protein kinase C-theta in Fas/Fas ligand-mediated
apoptosis. J. Immunol. 178, 312-319 (2007).
2. Kim, J K. et al., PKC-theta knockout mice are
protected from fat-induced insulin