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200-460-4
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关键词:'200-460-4'
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Spec Sheet: NovAseptic® Valve - NU######/460-321
diaphragm applied) mL Net Weight (approximate) kg (lb)
NU150050/460-321 84.0 1.7 (3.75)
NU150075/460-321 90.5 2.0 (4.41)
NU200100/460-321 230.0 3.5 (7.72)
Material
Bar
Stainless Steel in compliance
Product Information Sheet - CASP3F
fluorimeter: excitation – 360 nm
emission – 460 nm
slit width – 5 nm
4. Place 200 µl of each solution into the appropriate
microplate well.
5. Place 200 µl of 1× Assay Buffer into the blank well.
H2A.X (Ser139) Dual Detect CELISA Assay Kit (Fluorogenic Detection)
total antibody
fluorescence at 460 nm of the same well. The normalized triplicate readings for each
sample are then averaged.
Representative Data
0
2
4
6
Data Sheet - 53659
Typical precision (CV%): 4% at 0.25
mM
Equipment required but not included
Z805726-1EA FluoroSELECT™ Single channel
fluorometer ex 360 nm; em 460 nm
Z805823-100EA Glass vials for FluoroSELECT
Product Information Sheet - DNAQF
solution
(prepared in step 2) into the cuvette and place in
sample chamber.
4. Read the blank at 360 nm excitation and 460 nm
emission at ambient temperature. Using slits set at
2.5 nm for the 0.1–5
Data Sheet - D4060
chromatography.
Monoclonal Anti-DNA-PK reacts specifically with the
catalytic subunit of human DNA-PK (460 kDa). It may
be used for the detection of DNA-PK by immuno-
blotting, immunofluorescence, immunoprecipitation
Data Sheet - S0645
EFTSIGRSRIMGLSE
(PN1446-460), corresponding to amino acid residues
446-460 of rat PN1,1,2 with additional N-terminal
cysteine, as immunogen. The antibody was affinity
isolated on immobilized PN1446-460.
Anti-Sodium
MAK383 - Technical Bulletin
use as an
Uninduced Control.
3. Collect cells (1 × 106 cells)
by centrifugation.
4. Lyse cells in 200 L of chilled CD Cell
Lysis Buffer.
5. Incubate cells on ice for 10 minutes.
MAK374 Technical Bulletin
substrate solution to each well.
3. Mix well.
4. Monitor the fluorescence increase with a
fluorescence plate reader at λEx =
350 nm/, λEm = 460 nm; cutoff 420 nm.
For kinetic reading:
Product Information Sheet - A2027
released is detected by excitation at 370 nm and
emission at 460 nm. The fluorescence of
aminomethylcoumarin is unaffected by pH over the
range of pH 4 to pH 7.1
Precautions and Disclaimer
For Laboratory
MAK376- Technical Bulletin Final
substrate solution to each well.
3. Mix well.
4. Monitor the fluorescence increase with a
fluorescence plate reader at Ex = 350 nm/
Em = 460 nm; cutoff 660 nm.
For kinetic reading:
MAK373- Technical Bulletin Final
substrate
solution to each well.
3. Mix well.
4. Monitor the fluorescence increase with a
fluorescence plate reader at (Ex = 350 nm/
Em = 460 nm; cutoff 420 nm).
For kinetic reading:
Natrix® Q Chromatography Membrane
point pore diameter 1.4 μm
Typical membrane flow pore diameter 0.4 μm
Nominal unit membrane volume 460 mL
Typical unit flow rate range 2.3–11.5 L/min
Maximum operating pressure & temperature (liquids) 90
MOUSE ANTI-CILIARY NEUROTROPHIC FACTOR (CNTF)
1984).
2) J. Neurosci. 10:3507-3515 (1990).
3) J. Cell Biol. 118:139-148 (1992).
4) J. Cell Biol. 115:447-460 (1992).
5) Anal. Biochem. 112:195-203 (1981).
Important Note: During shipment
Product Information Sheet-LYSISO1
wavelength readings, the
same sample can be measured at 460 nm and
510 nm consecutively.
Calculation
Since the dye absorbance at 460 nm changes very
little during uptake, any change in this
ANTI-SODIUM CHANNEL Nav1.7, PAIN AFFINITY PURIFIED POLYCLONAL ANTIBODY
IMMUNOGEN: Purified peptide from rat Nav1.7, Pain (amino acids 446-460) (Accession O08562).
APPLICATIONS: Western blot: 1:200 on rat dorsal root ganglion.
Immunohistochemistry on rat dorsal root
Product Information Sheet-11449460001
July 2021
Fragment sizes: 72 to 1,353 bp
ΦX 174 DNA × Hae III digested.
Cat. No. 11 449 460 001 50 μg
200 μl
50 gel lanes
Store the product at −15 to
Product Information Sheet - MAK203
Substrate 2 µL
4. Add 25 µL of the Enzymatic Reaction Mix to each
reaction well. Mix well using a horizontal shaker or
by pipetting.
5. Measure the fluorescence (FLU, λex = 360/
λem = 460
Product Information Sheet - T9392
volume of 200 µl. The polymerization was
measured by an increase in absorbance at 350 nm
following a 30-second equilibration period.
References
1. Goedert, M.,Trends Neurosci, 16, 460-465 (1993
Product Information Sheet - MAK345
hydrolyzes the
non-fluorescent substrate to release a stable
fluorophore (ex = 380 nm/em = 460 nm). The kit
includes a selective chymotrypsin inhibitor that can be
used to measure specific chymotrypsin
Data Sheet - D7808
620 320 20 600
900 600 300 500
880 580 280 400
860 560 260 300
840 540 240 200
820 520 220 100
800 500 200
780 480 180
760 460 160
740 440 140
720 420 120
Components
Suitable for 50 applications of the
Product Information Sheet - MAK090
quenched substrate
L-γ-Glutamyl-7-amido-4-methylcoumarin (L-γ-Glutamyl-
AMC), liberating the fluorescent group, 7-Amino-
4-Methyl Coumarin (AMC) (λex = 365 nm/λem = 460 nm)
proportional to the GGT activity
MOUSE ANTI-ANGIOTENSIN-CONVERTING ENZYME (ACE) (CD143) MONOCLONAL ANTIBODY
. Immunol. (1994) 6:1153-1160.
10. PNAS.USA (1996) 93:5213-5218.
11. J. Nucl. Med. (1991) 32:453-460.
12. Lung (1992) 170:349-358.
13. J. Immunol. Methods. (1990) 132:263-273.
14. J. Hypertension (
A Neuronal and Astrocyte Co-Culture Assay for High Content Analysis of Neurotoxicity
bandwidth (nm)]
Emission Filter Range
[peak/bandwidth (nm)]
Hoechst HCS Nuclear Stain 20X 360/40 460/40 (or 535/50 if necessary)
HCS Secondary Antibody, FITC-Donkey
anti-Rabbit IgG
20X 480/40 535
Data Sheet - A1976
amyloid precursor protein: a potential toxic
mechanism in Alzheimer's disease. Nat. Neurosci.,
3, 460-464 (2000).
AH 10/02
Sigma brand products are sold through Sigma-Aldrich, Inc.
Cathepsins and Related Products Technical Bulletin
determination of cathepsin 219392 5 mg
Fluorogenic B activity. Excitations max.: ~380 nm; emission max.:~460 nm.
(Z-Arg-Arg-AMC, 2HCl)
Cathepsin D Substrate I 918.5 Excellent substrate for the quantitative determination
Product Information Sheet - MAK539
stored at 4 °C for future use.
Measurement
1. Add 200 µL of Reagent and tap lightly to mix.
2. Incubate for 1 minute at room temperature.
3. Read fluorescence emission at
Data Sheet - 53649
Material Required but not Provided:
FluoroSELECT™ single channel fluorometer (λex
360 nm; λem 460 nm)
Sterile rayon swab
Distilled H2O
Pipette and pipette tip
Precautions and Disclaimer
LT2 IMMORTALIZED PANCREATIC MESENCHYMAL CELL LINE - Data Sheet
centrifuge tube, add approximately 10 mLs of PBS
to the centrifuge tube, agitate and then centrifuge at 460 x g for 10 minutes. Decant the
supernatant as completely as possible and resuspend the cells in a
Data Sheet - 91333
Material Required but not Provided:
FluoroSELECT™ single channel fluorometer (λex
360 nm; λem 460 nm)
Sterile rayon swab
Distilled H2O
Pipette and pipette tip
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