activated by a 43-amino acid RFamide peptide, QRFP43, and a 26 amino acid fragment,
QRFP26/26RFa/Peptide 518 (Jiang et al., 2003; Fukusumi et al., 2003). QRFP43 is the
active form in rat brain, and intracerebral
forebrain with reference to tyrosine hydroxylase-
immunohistochemistry. Brain Res., (1990) 518, 83-94.
9) OKAMURA H., KITAHAMA K., MONS N., MATSUMOTO Y., IBATA Y. and GEFFARD M. Heterogeneous
distribution
Res. 18, 6069-6074.
2. Haun, R. S., Servanti, I. M., and Moss, J. (1992) BioTechniques 13, 515–
518.
3. Novy, R., Yaeger, K., Held, D., and Mierendorf, R. (2002) inNovations
15, 2–6.
Compatible
using
500 µL/well.
9. Dilute Donkey anti-Mouse IgG, Cy3 conjugate and DAPI in fluorescent staining buffer. Use
Donkey anti-Mouse IgG, Cy3 conjugate at a 1:200 dilution and DAPI at a 1:100 dilution
Human ECM345
MMP-2 Human ECM492
MMP-9 Human ECM494
TIMP-1 Human ECM496
TIMP-2 Human ECM498
http://www.emdmillipore.com/US/en/product/%2CMM_NF-17-518?cid=BI-XX-BRC-C-EPDF-MPX0-B053-1505
necrosis, cachexia, shock, and
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2. Glue, C. et al. LPS-induced cytokine production in the monocytic cell line
THP-2 determined
mixture to cool for 5 minutes.
8. Add 200 µL of 1X gelatin mixture to each well; incubate at room temperature for 10 minutes,
protected from light.
9. Remove gelatin mixture; rinse three times
total 1X gelatin
mixture.
8. Add 200 µL of 1X gelatin mixture to each well; incubate at room temperature for 10 minutes,
protected from light.
9. Remove gelatin mixture; rinse three times
R.E., et al. 1992. Am. J. Hum. Genet. 51,
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Bakker, E., et al. 1991. Am. J. Hum. Genet. 49, 518.
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cell lysates. Also detects a ~28 kDa band not detected by
the preimmune serum.
Anti-BACE2 (504-518), 195104 100 µl Recognizes the expected ~47 kDa band in guinea pig brain
Human (Rabbit) IB cell lysates
detection
method and uses a cell-permeable green fluorescent
Cyto-dye (Exc. max.: 488 nm; Em. max.: 518 nm) to stain
live cells, and propidium iodide (Exc. max.: 488 nm; Em.
max.: 615 nm) to stain dead
peripheral blood mononuclear cells
from dogs with canine demodicosis. J Vet Med Sci 2002; 64:
513–518.
26. Felix AOC, Guiot EG, Stein M et al. Comparison of systemic
interleukin 10 concentrations in