APPLICATIONS:
Western blot: 1:1000; the antibody is most effective under non-reducing conditions.
Immunohistochemistry: Frozen sections; 1:200-1:400. Paraffin-embedded kidney with
soluble
Ergonovine 3600 573 water soluble
Etoposide 3842 610 30% alcohol
Factor III 3873 616 water soluble
Factor IX 3874 616 water soluble
Fentanyl 3944 628 water soluble
Fluorouracil 4109 654 water soluble
soluble
Ergonovine 3600 573 water soluble
Etoposide 3842 610 30% alcohol
Factor III 3873 616 water soluble
Factor IX 3874 616 water soluble
Fentanyl 3944 628 water soluble
Fluorouracil 4109 654 water soluble
determined by a Scatchard
plot using this RIA system.
Ka = 1-10 x10
9 L/mole.
Bibliography
Koch, Y., et al., Biochem. Biophys. Res. Comm., 55,
616 (1970).
*Each vial contains no more than 20 mg Polyvinyl
protein solution in the collection tube.
Repeat wash once.
References
1. Carlson, D.M., and Blackwell, C., J. Biol. Chem.,
243, 616-626 (1968).
2. Lloyd, K.O. et al., J. Biol. Chem., 271, 33325-33334
carcinoma of the lung.
Hum. Mutat., 29, 609-616 (2008).
Precisio is a trademark of Sigma-Aldrich
Biotechnology LP and Sigma-Aldrich Co.
BKR,MAM 11/09-1
Sigma brand products are sold through Sigma-Aldrich
neuroendocrine carcinoma of the lung.
Hum. Mutat., 29, 609-616 (2008).
PRECISIO is a registered trademark of Sigma-Aldrich
Co. LLC.
AD,BKR,MAM 02/18-1
2018 Sigma-Aldrich Co. LLC. All rights reserved
, and Riesling wine
diluted in water 1:10 and then in acetoni-
trile 1:10 (bottom trace). Asterisks (*)
indicates impurities in standards. Detection
by UV at 200 nm using a 2.5 µL semi-micro
flow-cell
test. Adjust the
procedure according to your experiment.
Immunoprecipitation
1. Transfer up to 1 ml cell lysate (200-500 µg protein)
into an Eppendorf tube. The optimal cell lysate
volume is around
Adjust the
procedure according to your experiment.
Immunoprecipitation
1. Transfer up to 1 ml of cell lysate/sample (200-500
µg protein) into a microcentrifuge tube. The
optimal cell lysate volume
reaction. Adjust
the amounts according to your experiment.
Immunoprecipitation
1. Transfer up to 1 ml of cell lysate (200-500 µg of
protein) into an Eppendorf microcentrifuge tube.
The optimal cell
Roth, T.L. et al. Nature. 2018;559(7714):405-9.
7. de Bock, C. E. et al. Cancer discovery. 2018;8(5):616-31.
8. Michael, P. M. et al. eLife 2019;8:e46314
. Add 200 μL of Phenol:Chloroform:Isoamyl Alcohol (25:24:1, pH 8.0) per sample.
56. Vortex the tube vigorously and centrifuge at 16,000 x g for 5 minutes at room temperature.
57. Add 200 μL of
neuroblastoma
with monoclonal antibodies HMB-45 and anti-synaptophysin. Arch
Pathol Lab Med. 112: 616-620 (1988).
3. Ordóñez N G, Xiaolong J and Hickey R C. Comparison of
HMB-45 monoclonal antibody and