PreACT
TM
Activated Resins
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Table of Contents
PreACT Activated
appropriate
column. Wash with 5-10 column volumes of PBS.
2. Apply the sample that contains the biotinylated
protein.
3. Wash with PBS until the absorbance at 280 nm is
minimal.
4.
5. Equilibrate column with 5 column volumes of 1X Ni-NTA Bind Buffer.
The flow rate should not exceed 2 ml/min.
Monitor elution at 280 nm; the baseline should be stable after washing with 5 column
).
16. Mitchel, R. E., et al., J. Biol. Chem., 245, 3485-
3492 (1970).
17. J. Biol. Chem., 207, 533 (1954).
18. Sluyterman, L. A., and de Graaf, M.J., Biochim.
Biophys. Acta, 258, 554-561 (
, Johansen, L., Schmid, R., Ugelstand,
J., Ellingsen, T. and Berge, A. Agents Actions
Suppl., 9, 207-212, (1982).
21. Tenoso, H.J., Smith, D.B., Covalent Bonding of
Antibodies to Polystyrene Latex
, Johansen, L., Schmid, R., Ugelstand,
J., Ellingsen, T. and Berge, A. Agents Actions
Suppl., 9, 207-212, (1982).
21. Tenoso, H.J., Smith, D.B., Covalent Bonding of
Antibodies to Polystyrene Latex
, Johansen, L., Schmid, R., Ugelstand,
J., Ellingsen, T. and Berge, A. Agents Actions
Suppl., 9, 207-212, (1982).
21. Tenoso, H.J., Smith, D.B., Covalent Bonding of
Antibodies to Polystyrene Latex
, Johansen, L., Schmid, R., Ugelstand,
J., Ellingsen, T. and Berge, A. Agents Actions
Suppl., 9, 207-212, (1982).
21. Tenoso, H.J., Smith, D.B., Covalent Bonding of
Antibodies to Polystyrene Latex
, Johansen, L., Schmid, R., Ugelstand,
J., Ellingsen, T. and Berge, A. Agents Actions
Suppl., 9, 207-212, (1982).
21. Tenoso, H.J., Smith, D.B., Covalent Bonding of
Antibodies to Polystyrene Latex
, Johansen, L., Schmid, R., Ugelstand,
J., Ellingsen, T. and Berge, A. Agents Actions
Suppl., 9, 207-212, (1982).
21. Tenoso, H.J., Smith, D.B., Covalent Bonding of
Antibodies to Polystyrene Latex
, Johansen, L., Schmid, R., Ugelstand,
J., Ellingsen, T. and Berge, A. Agents Actions
Suppl., 9, 207-212, (1982).
21. Tenoso, H.J., Smith, D.B., Covalent Bonding of
Antibodies to Polystyrene Latex