induced into apoptosis with 0.5 µM staurosporine for 5 hours (B) and fixed in cold
acetone for 5 minutes. Cells were incubated with PARP (214/215) CSSA at 10 μg/mL, then incubated with
biotinylated goat
peptide corresponding to residues 197-215 of rat β2 subunit of voltage-gated
Na+ channels (Accession number P54900).
APPLICATIONS: Western blotting: 1:200 on rat brain membranes.
Immunohistochemistry
Dimensions Total length: 320 mm (12.6 in.)
Length of lamp housing: 215 mm (8.5 in.)
Length of cable to connect to Elix® or
RiOs™ system: 5 m (16.4 ft.)
Install and UV lamp replacement requires
250
Preparation Instructions
Sodium arachidonate is soluble in water (5 mg/ml). It
is also soluble in ethanol (50 mg/ml) and methanol
(200 mg/ml).
Storage/Stability
If the sealed ampule of sodium arachidonate
purified peptide corresponding to residues 197-215 of rat β2 subunit of voltage-
gated Na+ channels (Accession P54900).
APPLICATIONS: Western blotting: 1:200 using ECL on rat brain membranes.
Immunohistochemistry
binding domain, and its C-terminal 89 kDa catalytic domain.
Cleavage occurs between Asp 214 and Gly 215
SPECIFICITY:
AB3620 detects only the cleaved large 89kDa subunit of human PARP and does
et al., J. Biol. Chem., 268, 1125 (1993).
5. Jones, J., et al., Proc. Nat. Acad. Sci. USA, 88,
7481 (1991).
6. Koistinen, R., et al., Clinica Chimica Acta, 215, 189
(1993).
BIOHAZARD: Handle as
et al., J. Biol. Chem., 268, 1125 (1993).
5. Jones, J., et al., Proc. Nat. Acad. Sci. USA, 88, 7481
(1991).
6. Koistinen, R., et al., Clinica Chimica Acta, 215, 189
(1993).
BIOHAZARD: Handle as
Enzyme Activity and Solubilization of Lipid Bilayer
Membranes. Biochim. Biophys. Acta, 733(2), 210-
215 (1983).
3. Protein Structure: A Practical Approach, 2nd ed.,
Creighton, T. E., ed., IRL Press at
contains a concentration of 90 mM Tris-HCl buffer, 8.5 mM CaCl2 ,
5 mM DTT, 0.5 mM EDTA, pH 7.6.
The recommended amount of enzyme is 1/200 to 1/50 of the protein by weight.
Enzyme Activity and Solubilization of Lipid Bilayer
Membranes. Biochim. Biophys. Acta, 733(2), 210-
215 (1983).
3. Protein Structure: A Practical Approach, 2nd ed.,
Creighton, T. E., ed., IRL Press at
Enzyme Activity and Solubilization of Lipid Bilayer
Membranes. Biochim. Biophys. Acta, 733(2), 210-
215 (1983).
3. Protein Structure: A Practical Approach, 2nd ed.,
Creighton, T. E., ed., IRL Press
been published.9 The oxidation index is the ratio
of the absorbance at 233 nm to the absorbance at 215
nm. The latter wavelength was chosen since there is
little contribution of the fatty acid carbonyl
John Wiley & Sons, Inc., New
York,1999).
5. Kuehl, F.A., Jr. et al., J. Am. Chem. Soc., 77, 2344
(1955).
6. Sirvio, J., et al., Neurosci. Lett., 146, 215 (1992).
7. Ohno, M. and Watanabe, S., Eur.
John Wiley & Sons, Inc., New
York,1999).
5. Kuehl, F.A., Jr. et al., J. Am. Chem. Soc., 77, 2344
(1955).
6. Sirvio, J., et al., Neurosci. Lett., 146, 215 (1992).
7. Ohno, M. and Watanabe, S., Eur.
John Wiley & Sons, Inc., New
York,1999).
5. Kuehl, F.A., Jr. et al., J. Am. Chem. Soc., 77, 2344
(1955).
6. Sirvio, J., et al., Neurosci. Lett., 146, 215 (1992).
7. Ohno, M. and Watanabe, S., Eur.
glycerol.
Molecular mass: ∼108 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 159–215 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This product is for R&D use only, not for