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Christine E Quartararo et al.
Biochemistry, 52(51), 9286-9293 (2013-11-23)
Isocitrate lyase (ICL) catalyzes the reversible retro-aldol cleavage of isocitrate to generate glyoxylate and succinate. ICL is the first enzyme of the glyoxylate shunt, which allows for the anaplerosis of citric acid cycle intermediates under nutrient limiting conditions. In Mycobacterium
Jean-Paul Meijnen et al.
The Journal of biological chemistry, 287(18), 14606-14614 (2012-03-15)
Previously, an efficient D-xylose utilizing Pseudomonas putida S12 strain was obtained by introducing the D-xylose isomerase pathway from Escherichia coli, followed by evolutionary selection. In the present study, systemic changes associated with the evolved phenotype were identified by transcriptomics, enzyme
Alessio Aprile et al.
BMC genomics, 14, 821-821 (2013-11-26)
Durum wheat often faces water scarcity and high temperatures, two events that usually occur simultaneously in the fields. Here we report on the stress responsive strategy of two durum wheat cultivars, characterized by different water use efficiency, subjected to drought
Eric Nurit et al.
Analytical and bioanalytical chemistry, 407(12), 3471-3479 (2015-02-24)
Wheat is the second largest crop cultivated around the world and constitutes a major part of the daily diet in Europe. It is therefore important to determine the content of micronutrient in wheat and wheat-based food products to define the
E D Booth et al.
Xenobiotica; the fate of foreign compounds in biological systems, 34(1), 31-48 (2004-01-27)
1. The metabolism of [1,2-(14)C]-ethylene glycol and [1,2-(14)C]-glycolic acid was studied in vitro using precision-cut tissue slices prepared from the livers of female Sprague-Dawley rats, New Zealand white rabbits and humans. The time-course for production of metabolites formed from ethylene
Ruth Belostotsky et al.
Journal of molecular medicine (Berlin, Germany), 90(12), 1497-1504 (2012-06-26)
Perturbations in glyoxylate metabolism lead to the accumulation of oxalate and give rise to primary hyperoxalurias, recessive disorders characterized by kidney stone disease. Loss-of-function mutations in HOGA1 (formerly DHDPSL) are responsible for primary hyperoxaluria type III. HOGA1 is a mitochondrial
Kelly C Fahnoe et al.
PloS one, 7(12), e51732-e51732 (2012-12-15)
Antibacterial compounds that affect bacterial viability have traditionally been identified, confirmed, and characterized in standard laboratory media. The historical success of identifying new antibiotics via this route has justifiably established a traditional means of screening for new antimicrobials. The emergence
P E Borondy et al.
Drug metabolism and disposition: the biological fate of chemicals, 12(4), 444-451 (1984-07-01)
Isoxicam a new nonsteroidal antiinflammatory agent was radiolabeled with 14C at the 3-position of the benzothiazine nucleus. It was well absorbed following peroral administration to man, monkey, dog, and rat, reaching peak plasma concentrations in 4-8 hr. Over 90% of
Saurabh Awasthi et al.
Applied biochemistry and biotechnology, 177(5), 1013-1024 (2015-08-16)
Increased burden of advanced glycation end-products (AGEs) in case of hyperglycemic conditions leads to the development of retinopathy, nephropathy, and cardiovascular and neurological disorders such as Alzheimer's disease. AGEs are considered as pro-oxidants, and their accumulation increases the oxidative stress.
E Mentasti et al.
Journal of chromatography, 417(2), 253-260 (1987-07-03)
A high-performance liquid chromatographic method for the determination of carbonyl compounds, namely aldehydes, ketones and keto acids in urine, has been developed. Pre-column derivatization with 2,4-dinitrophenylhydrazine offers sufficient sensitivity for the determination of glyoxylic acid in urine with a detection
Sang-Jin Cho et al.
Journal of nanoscience and nanotechnology, 11(6), 5328-5333 (2011-07-21)
Microwave (MW) plasma was applied to the surface of polyimide (PI) films as a treatment to enhance the adhesion between copper deposition layer and PI surface for electroless plating. The influences of nitrogen MW plasma treatment on chemical composition of
Venkata S K Indurthi et al.
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences, 59, 58-68 (2014-04-23)
Glycation alters the drug binding properties of serum proteins and could affect free drug concentrations in diabetic patients with elevated glycation levels. We investigated the effect of bovine serum albumin glycation by eight physiologically relevant glycation reagents (glucose, ribose, carboxymethyllysine
Xueyang Feng et al.
PLoS computational biology, 8(2), e1002376-e1002376 (2012-02-10)
Shewanella oneidensis MR-1 sequentially utilizes lactate and its waste products (pyruvate and acetate) during batch culture. To decipher MR-1 metabolism, we integrated genome-scale flux balance analysis (FBA) into a multiple-substrate Monod model to perform the dynamic flux balance analysis (dFBA).
W L Davis et al.
The Anatomical record, 234(4), 461-468 (1992-12-01)
The enzymatic activities unique to the glyoxylate cycle of higher plants and certain lower invertebrates, isocitrate lyase and malate synthase, have been demonstrated in homogenates prepared from human liver. Human liver can also carry out cyanide-insensitive fatty acid oxidation from
Yi Li et al.
Organic letters, 14(8), 2062-2065 (2012-04-07)
An efficient and straightforward method for the preparation of highly enantiomerically enriched β,γ-unsaturated α-amino acid derivatives by a Lewis acid promoted diastereoselective Petasis reaction of vinylboronic acid, N-tert-butanesulfinamide, and glyoxylic acid has been developed. The synthetic utilities of the approach
Myung-Il Kim et al.
PloS one, 7(12), e52066-e52066 (2013-01-04)
Nitrogen metabolism is one of essential processes in living organisms. The catabolic pathways of nitrogenous compounds play a pivotal role in the storage and recovery of nitrogen. In Escherichia coli, two different, interconnecting metabolic routes drive nitrogen utilization through purine
Norio Sakai et al.
Organic letters, 14(3), 836-839 (2012-01-27)
A novel copper-catalyzed [5 + 1] annulation of 2-ethynylanilines with an N,O-acetal, which functioned as a C1 part, leading to the preparation of quinoline derivatives with an ester substituent on the 2-position is described. A combination of CuBr(2) and trifluoroacetic
Antonio Di Matteo et al.
Biochemical genetics, 50(11-12), 908-921 (2012-08-23)
In recent years, interest in tomato breeding for enhanced antioxidant content has increased as medical research has pointed to human health benefits from antioxidant dietary intake. Ascorbate is one of the major antioxidants present in tomato, and little is known
Kohki Chihara et al.
Analytical and bioanalytical chemistry, 403(9), 2765-2770 (2012-05-15)
The Petasis reaction is the multi-component reaction of a carbonyl compound, amine, and arylboronic acid to form an α-amino acid or a β-aminoalcohol. In this work, as the first analytical application of the Petasis reaction, a high-performance liquid chromatographic (HPLC)
Jean-Paul Otamonga et al.
Luminescence : the journal of biological and chemical luminescence, 30(5), 507-511 (2014-09-17)
In order to study the mechanism of the enhancement of solution chemiluminescence, the kinetics of the decay of the oxidant and the chemiluminescence emission were followed for oxidations by permanganate, manganese dioxide sol and Mn(3+) (aq) of glyoxylic acid, using
Vijai Bhadauria et al.
PloS one, 7(4), e36266-e36266 (2012-05-05)
The role of β-oxidation and the glyoxylate cycle in fungal pathogenesis is well documented. However, an ambiguity still remains over their interaction in peroxisomes to facilitate fungal pathogenicity and virulence. In this report, we characterize a gene encoding an alanine
F J Bruzzese et al.
Biomaterials, artificial cells, and artificial organs, 18(2), 143-156 (1990-01-01)
Hemoglobin obtained from out-dated human blood was stripped of 2,3-diphosphoglycerate and modified with the crosslinking agents glyoxalic acid, 1,2-cyclohexadione, or fumarate to stabilize the tetramer. The resulting hemoglobins, which show alterations in their oxygen transport capability, have been studied in
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