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405-18-5
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关键词:'405-18-5'
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EX-CELL 405 Serum-Free Medium for Insect Cells
disturbing the cell pellet.
4. Resuspend the cells in 5 mL of EX-CELLTM 405 medium.
5. Count the cells for viability and transfer to a sterile shaker
flask at a seeding density of 2-4 x 105 cells/
Protocol-927074
Procedure
A. Prepare bioink
1. Reconstitute TissueFab® bioink Fibrin-Vis/405 nm by adding 5 ml buffer component to each 5 g ink powder
component. Put the reconstituted ink in a water bath
Insect Expression Products EX-CELL Serum-Free Media for Insect Cells
Biosciences Pty. Ltd.
18-20 Export Drive
Brooklyn, Victoria 3025
AUSTRALIA
Phone +61 (0)3-9362-4500
Toll free-AUS 1 800-200-404
Fax +61 (0)3-9315-1656
E-mail info-ap@sial.com
EX-CELL™ 405
Serum-Free Medium
Product Information Sheet - SE120140
170±10 rpm for 4 hours±30 minutes
at room temperature (18–26 °C).
6. First aspirate the fluid completely and then
wash/aspirate each well five (5) times with the
Working Wash Solution using an automatic
925055 Protocol
Profile of TissueFab® bioink Alg(Gel)ma Vis/405 nm
Figure 5. Mechanical application data for the crosslinking profile for TissueFab® bioink Alg(Gel)ma Vis/405 nm,
measured using TA Instruments Discovery
SMC®Plate Washer Evaluation Kit
68 51 56 54 55 68 62 83 61 11 18
H 97 76 89 77 80 83 85 71 87 88 78 81 83 7 9
Column Mean 78 65 72 63 69 67 65 63 64
LC-MS Contaminants: Avoid, identify, mimimize.
101R Detergents
383.2797 [AB4+H]+ [C14H22O][C2H4O]n Triton® X-100, X-114, X-405, or X-45 Detergents
388.1284 [M+NH4]+ [C2H6SiO]5 Polysiloxane Polysiloxane, (see m/z 371)
389.2515 [A6B+Na]+ [C3H6O]nH2O PPG
Product Information Sheet - SE120107
provided (i.e., 1, 2, 3, 4, and 5). For
the 405 nm readings, construct a second dose
response curve using the three calibrators with the
highest concentrations (i.e., 4, 5, and 6).
2. Assign the concentration
EX-CELL 420 Frequently Asked Questions
T015.qxd
How does EX-CELL™ 420 differ from EX-CELL™ 405?
EX-CELLTM 405 is quite different in terms of the lipids, sugars,
hydrolysate concentration, amino acids and vitamins.
Can I use EX-CELL
Product Information Sheet - P5744
yellow in color
and can be read spectrophotometrically at 405 nm.
The pNPP reaction may be stopped with 3 M NaOH
solution and read at 405 nm.
This product consists of capsules formulated with
DataSheet -P5869
yellow in color
and can be read spectrophotometrically at 405 nm.
The pNPP reaction may be stopped with 3 M NaOH
solution and read at 405 nm.
This product consists of capsules formulated with
Product Information Sheet - N9389
in color and can be read
spectrophotometrically at 405 nm. The pNPP reaction
may be stopped with the addition of 3 M NaOH
solution and read at 405 nm.
The N9389 tablets are supplied as 50
Brochure: LC-MS Contaminants
101R Detergents
383.2797 [AB4+H]+ [C14H22O][C2H4O]n Triton® X-100, X-114, X-405, or X-45 Detergents
388.1284 [M+NH4]+ [C2H6SiO]5 Polysiloxane Polysiloxane, (see m/z 371)
389.2515 [A6B+Na]+ [C3H6O]nH2O PPG
Product Information Sheet - N2765
color and can be read
spectrophotometrically at 405 nm. The pNPP reaction
may be stopped with the addition of 3 M NaOH
solution and read at 405 nm.
The N2765 tablets are supplied as
Product Information Sheet - P3233
optimal signals (absorbance at 405 nm of
approximately 1 for 100 ng standards). If so, be
sure that the incubation times for the samples and
standards are identical.
18. Stop the color development
DataSheet - S0942
read spectrophotometrically at 405 nm.
The pNPP reaction may be stopped with 3 M NaOH
solution and read at 405 nm.
This product consists of formulated tablets with 5 mg
of pNPP per individual tablet
Product Information Sheet - MAK461
an intense
yellow soluble product under alkaline
conditions and can be conveniently measured
at 405 nm on a spectrophotometer.
The pNPP Phosphatase Assay kit involves
adding a single reagent to the
DataSheet -P5744
yellow in color
and can be read spectrophotometrically at 405 nm.
The pNPP reaction may be stopped with 3 M NaOH
solution and read at 405 nm.
This product consists of capsules formulated with
DataSheet - P4744
yellow in color
and can be read spectrophotometrically at 405 nm.
The pNPP reaction may be stopped with 3 M NaOH
solution and read at 405 nm.
Several theses4 dissertations5-10 have cited use of
Product Information Sheet-N2640
color and can be read
spectrophotometrically at 405 nm. The pNPP reaction
may be stopped with the addition of 3 M NaOH
solution and read at 405 nm.
The N2640 tablets are supplied as
Product Information Sheet - N1891
period, read the plate at
405 nm on a multiwell plate reader.
4. If the plate cannot be read immediately, add 50 L
of 3 N NaOH solution per 200 L of reaction
mixture.
Protocol-927066
GelHA)ma -Vis/405 nm 919624
TissueFab® - bioink (Gel)ma -VIS/405nm, low endotoxin 918741
TissueFab® - bioink (GelAlg)ma -UV/365 nm 920983
TissueFab® - bioink (GelAlg)ma -Vis/405 nm 921610
Product Information Sheet - SE120080
Enzyme labeled antibody)
to each well.
5. Cover the plate with aluminum foil to avoid
exposure to light and Incubate for 4 hours at room
temperature (18–26 °C) with shaking.
6. Remove liquid from
DataSheet - P5994
yellow in color
and can be read spectrophotometrically at 405 nm.
The pNPP reaction may be stopped with 3 M NaOH
solution and read at 405 nm.
This product consists of formulated tablets with
40
Data Sheet - SAB4600023
photostability, and/or specificity. CF405S is spectrally
similar to Alexa Fluor 405, Cascade Blue, and
DyLight 405 (see Figure 1).
Figure 1.
Absorption/Emission Spectra of CF405S Conjugated
Antibodies
Data Sheet - SAB4600024
photostability, and/or specificity. CF405S is spectrally
similar to Alexa Fluor 405, Cascade Blue, and
DyLight 405 (see Figure 1).
Figure 1.
Absorption/Emission Spectra of CF405S Conjugated
Antibodies
Product Information Sheet - CASP8C
ELISA reader and measure the
initial absorbance at 405 nm (t = 0) and then
read at 5 minute intervals for t minutes (where t
can be from 20-60 minutes or even longer for
very dilute samples).
Product Information Sheet - N2770
period, read the plate at
405 nm on a multiwell plate reader.
4. If the plate cannot be read immediately, add 50 L
of 3 N NaOH solution per 200 L of reaction
mixture.
Product Information Sheet-10745731001
much higher SH values
will be found.
2.2. Parameters
Absorption
Absorption coefficient at 405 nm:
2-nitrophenol = 3.5 [l × mM-1 × cm-1 ]
4-nitrophenol = 18.5 [l × mM-1 × cm-1 ]
EC-Number
MultiScreen Ultracel®-10 filter plate: Enzymatic Activity Recovery
.0 µl
6. Read plate immediately on SpectraMax plate reader at 405-nm wavelength, acquiring
readings at 0, 1, 2, 3, 4 and 5 minutes at 37°C.
7. Calculate Alkaline Phosphatase activity by obtaining
1/20