488-17-5 | Sigma-Aldrich

Fluorescent Mouse ES/iPS Cell Characterization Kit

TRA-1-81, Alexa Fluor® 488 conjugate conjugate, 100 µL (Cat. No. MAB4381A4) 16. Anti-DPPA-2, clone 6C1.2, Alexa Fluor® 488 conjugate, 100 µL (Cat. No. MAB4356A4) 17. Anti-DPPA-2, clone 6C1.2

Fluorescent Human ES/iPS Cell Characterization Kit

One vial containing 50 µL of 0.5 mg/mL conjugated monoclonal antibody. 5. Mouse anti-Nanog, clone 7F7.1, Alexa Fluor® 488 conjugate (Part No. MABD24A4-50UL). One vial containing 50 µL of 0.5 mg

Data Sheet - N6288

antibody to Atto 488-NHS (λex 500 nm; λem 522 nm), Catalog Number 41698, and the conjugate is purified by gel filtration to remove unbound Atto 488-NHS fluorophore. Anti-Nucleolin−Atto 488 recognizes human

EdU Cell Proliferation Assay

EdU Cell Proliferation Assay EdU-488 Catalog No. 17-10525 EdU-555 Catalog No. 17-10526 EdU-594 Catalog No. 17-10527 EdU-647 Catalog No. 17-10528 100 reactions FOR

BioFiles-Issue

g m a - a l d r i c h . c o m 5 Atto 465 449 503 55 75000 Ar laser Atto 488 501 523 80 90000 Ar laser FITC, Alexa 488 Atto

FlowCellect™ p38 Stress Pathway Activation Detection Kit

pipetting up and down. 13. Add 5 μL of 20X antibody to tubes using the flowing chart. Tube # Description 1 Treated cells stained with pP38(T180/Y182)-AlexaFluor®488 2 Treated cells stained with

Low Background Membrane for Fluorescent Protein Detection in Western Blotting

Fluorescein, Oregon Green 488, Cy2, Fluoro Emerald, Alexa Fluor 488 Green 492 535 1209 11688 1880 Fluorescein, Oregon Green 488, Oregon Green 514, Alexa Fluor 514, Alexa Fluor 488 Red-Orange 485 595

FlowCellect™ STAT3 Activation Dual Detection Kit (FCCS025143)

and add 5 µL of either Anti-phospho-STAT3-Alexa Fluor® 647 or Anti-STAT3-Alexa Fluor® 488 to each sample well. 20. For multiplexing, resuspend the cells in 90 µL of assay buffer and add 5 µL of Anti-phospho

Product Information Sheet-peps-ro

2019 Lyophilized Cat. No. 10 253 286 001 2 mg Cat. No. 11 359 053 001 10 mg Cat. No. 11 524 488 001 50 mg Store the lyophilizate at +2 to +8°C.

FlowCellect™ STAT1 Activation Dual Detection Kit

buffer and add 5 µL of either Anti-phospho-STAT1-Alexa Fluor® 488 or Anti-STAT1-PerCP to each sample well. 17. For multiplexing, resuspend the cells in 90 µL of assay buffer and add 5 µL of Anti-phospho

Product information sheet-P9290

., Nature, 541(7638), 488-493 (2017). 15. Eyal, S. et al., Development, 146(14), dev167882 (2019). 16. Zuo, Y. et al., PLoS ONE, 14(10), e0223299 (2019). 17. Lai, J.-J. et al.

FlowCellect™ Bcl-2 Activation Dual Detection Kit

pipetting up and down. 16. Add 5 μL of 20X antibody to tubes using the flowing chart. Tube # Description 1 Treated cells stained with Bcl-2 AlexaFluor®488 2 Treated cells stained with

FlowCellect™ Src Activation Dual Detection Kit (25 Tests)

buffer and add 5 µL of either Anti-phospho-Src-Alexa Fluor 488 or Anti-Src-Alexa Fluor 647 to each sample well. 22. For multiplexing, resuspend the cells in 90 µL of assay buffer and add 5 µL of Anti-phospho

FlowCellect™ PLC-g1 Activation Dual Detection Kit (25 Tests)

buffer and add 5 µL of either Anti-phospho-PLC-γ1-Alexa Fluor 488 or Anti-PLC-γ1-PE to each sample well. 22. For multiplexing, resuspend the cells in 90 µL of assay buffer and add 5 µL of Anti-phospho

FlowCellect™ Histone H2A.X Phosphorylation Assay Kit 100 Tests

again. 5 III. Cell Staining and Flow Analysis 23. Resuspend the cells in 95 µL of Assay Buffer and add

Data Sheet - SAB4200718

13. Eslami M., et al., Cell J., 17, 478-488 (2015). 14. Wagenseil J. and Mecham RP., J Cardiovasc Transl Res., 5, 264-73 (2012). SG,DR_OKF/LV,PHC 03/17-1

Data Sheet - A2980

2519-2529 (2001). 5. Lawrence, S.D., et al., BioTechniques, 35, 488-492 (2003). 6. Blissard, G.W. ,and Rohrmann, G.F., Virology, 170, 537-555 (1989). DS,PHC 01/17-1

Product Information Sheet - M2128

responsible for formazan patterns in dehydrogenase histochemistry. Experientia, 17, 136 (1961). 10. J. Histochem. Cytochem., 5, 515 (1957). 11. Bernofsky, C., and Swan, M., An improved cycling assay

BioTracker¿ NIR694 Nuclear Dye (Water)

Dye (Water) is a far red nuclear stain for live cells. The dye can be excited by wavelengths from 488 to 647nm and emits far red fluorescence with emission maximum at 694nm. The dye specifically stains

Data Sheet - U4002

in inhibition by beta-mercaptoethanol, phosphoramidate, and fluoride. Can. J. Biochem., 58, 481-488 (1980). 6. Dixon, N.E. et al., Metal ions in enzymes using ammonia or amides. Science, 191, 1144

Data Sheet - U1875

in inhibition by beta-mercaptoethanol, phosphoramidate, and fluoride. Can. J. Biochem., 58, 481-488 (1980). 6. Dixon, N.E. et al., Metal ions in enzymes using ammonia or amides. Science, 191, 1144

Data Sheet - U0251

in inhibition by beta-mercaptoethanol, phosphoramidate, and fluoride. Can. J. Biochem., 58, 481-488 (1980). 6. Dixon, N.E. et al., Metal ions in enzymes using ammonia or amides. Science, 191, 1144

Data Sheet - U1500

in inhibition by beta-mercaptoethanol, phosphoramidate, and fluoride. Can. J. Biochem., 58, 481-488 (1980). 6. Dixon, N.E. et al., Metal ions in enzymes using ammonia or amides. Science, 191, 1144

Product Information Sheet - APPA008

Table 1. Fluorescence wavelengths Fluorescent protein λexcitation λemission GFP 488 nm 525 nm CFP 488 nm 500 nm YFP 488 nm 550 nm Note: Emission wavelengths can be ±25 nm Results Subtract the blank

Product Information Sheet - APPA001

Table 1. Fluorescence wavelengths Fluorescent protein λexcitation λemission GFP 488 nm 525 nm CFP 488 nm 500 nm YFP 488 nm 550 nm Note: Emission wavelengths can be ±25 nm Results Subtract the blank

Product Information Sheet - APPA009

Table 1. Fluorescence wavelengths Fluorescent protein λexcitation λemission GFP 488 nm 525 nm CFP 488 nm 500 nm YFP 488 nm 550 nm Note: Emission wavelengths can be ±25 nm Results Subtract the blank

FlowCellect™ PI3K-mTOR Signaling Cascade Mapping Kit

treated and untreated), add 5 μL 20X Anti-phospho- Ribosomal Protein S6 (Ser235) PerCP conjugate Monoclonal Antibody and 5 μL 20X Anti- phospho-Akt1/PKBα (Ser473) Alexa Fluor 488 conjugate Monoclonal Antibody

Product Information Sheet - PISTAIN

2. Place white blood cells in clean 17 x 100 mm tube. 3. Resuspend cells in 5 ml citrate buffer solution. 4. Centrifuge for 5 minutes (300 x g) at room temperature. 5. Aspirate supernatant and resuspend

FlowCellect™ Multi-Color DNA Damage Response Kit

and down. 16. Add 5 μL(Guava) or 10 μL(Other) of 20X antibody to tubes using the flowing chart. Tube # Description 1 Untreated cells stained with pSMC1-AlexaFluor®488 2 Treated cells stained

Cytoskeletal Signaling Antibodies, Reagents and Kits

kit 17-455 STAR Phospho-MEK1 (Ser218/Ser222) ELISA Kit ELISA 96 well 1 kit 17-456 MEK1 Assay Kit KA 1 kit 17-157 MEK1 cDNA (wt) in pUSEamp TFX 5 µg 21-106 MEK2 cDNA (wt) in pUSEamp TFX 5