CD31 Ms-Cy5
SmartFlare™
RNA Detection Probe
Cat. # SF-492
pack size: 50µL (250 rxns)
Store at 2-8°C,
after reconstitution store at 23-27°C
DO NOT FREEZE
months at
2–8 C.
3
At pH 4–11.5, solutions containing Ca
2+
(1–6 mM) are expected to remain active for several
weeks. An 80% ammonium sulfate suspension stored
at 2–8
expression levels in gastrointestinal tissues.
This COX-1 product is supplied as a solution in 80 mM
Tris-HCl, pH 8, with 0.1% TWEEN 20 and 300 µM
diethyldithiocarbamate (DDC) as a preservative.
Note: DDC
months at
2–8 C.
3
At pH 4–11.5, solutions containing Ca
2+
(1–6 mM) are expected to remain active for several
weeks. An 80% ammonium sulfate suspension stored
at 2–8
components
RBD and Biotin-ACE2.
• Plate reader equipped with a 450 nm filter
Optional: with a 492 nm filter for end point
reading (upon addition of 3M HCl or 3M H2SO4)
• 37
least 12 months at
2–8 °C.3 At pH 4–11.5, solutions containing Ca2+
(1–6 mM) are expected to remain active for several
weeks. An 80% ammonium sulfate suspension stored
at 2–8 °C remains active for
Measure at 405 nm against ABTS substrate solution as a blank; reference wavelength approximately 492 nm.
The green color of the ABTS substrate can be easily detected by eye; for numeric values, a photometric
Measure at 405 nm against ABTS substrate solution as a blank; reference wavelength approximately 492 nm.
The green color of the ABTS substrate can be easily detected by eye; for numeric values, a photometric
Measure at 405 nm against ABTS substrate solution as a blank; reference wavelength approximately 492 nm.
The green color of the ABTS substrate can be easily detected by eye; for numeric values, a photometric
with
appropriate configuration for fluorescein. FITC has
an absorption maximum at approximately 492 nm
with an emission maximum at 520 nm.
Note: The fluorescence properties of FITC and FITC
conjugates
carried out without using stop solution and then
measured at 370 nm (measuring wavelength) against 492 nm (reference wavelength).
However, the absorbance values will be 25 –30% lower.
Reagents of different
spectrophotometrically at 450 nm. The OPD reaction
may be stopped with 3 M HCl or 3 M H2SO4 solution,
and read at 492 nm.
NH2
NH2
Peroxidase
NH2
NH2
N
N
References
1. Engvall, E., Enzyme Immunoassay ELISA and
EMIT. Methods in Enzymology, 70, 419- 492
(1980).
2. Uotila, M. et al., Two-site sandwich enzyme
immunoassay with monoclonal antibodies
polyvinyl alcohol with DABCO. Examination of the cells was performed by
fl uorescence microscopy at 492/520 nm. Alternatively, the cells were also stained with DAPI at a
dilution of 1/5,000 and examined
5–75 nmole/well). Tyrosine is determined
by measuring a colorimetric product with absorbance at
492 nm (A492) proportional to the amount of tyrosine
present.
Components
The kit is sufficient for
Pharmaceutics, 2015. 492 (1-2): p. 1–9.
7 Zheng M., et al., Polyvinyl Alcohol in Hot Melt Extrusion to Improve
the Solubility of Drugs. European Pharmaceutical Review, 2017(3).
8 Kasselkus A., et
solution.
Without TMB stop solution (Bottle 8): measure the absorbance of the samples in an ELISA reader at 370 nm
(reference wavelength approximately 492 nm).
Not stopping the substrate reaction allows
Color will begin to fade after 5 minutes.
If you want to measure at 370 nm (reference wavelength 492 nm):
– Do not add stop solution.
– Measure the absorbance at specific time points after substrate