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resazurin for the final 16-20 hours. The fluorescence
was then read in a microplate plate reader set at
544/590 nm.
The ND50 is the concentration of antibody required to
yield one-half maximal inhibition
cytochrome
P450c gene expression by cotransfection with
adenovirus E1A DNA. Eur. J. Biochem., 181, 539-
544 (1989).
12. Stein, R., and Ziff, E.B., Repression of insulin gene
expression by adenovirus type
cytochrome P450c
gene expression by cotransfection with adenovirus
E1A DNA. Eur. J. Biochem., 181, 539-544 (1989).
14. Stein, R., and Ziff, E.B., Repression of insulin gene
expression by adenovirus type
pg/pmole = 5755 pg/µL
References
1. Talayero, B.G, and Sacks, F.M., The role of
triglycerides in atherosclerosis. Current Cardiology
Reports, 13(6), 544-52 (2011).
4
Troubleshooting
, O., et al., PNAS (2005) 102:5227-5232.
66) Frappart, P., et al., Nature Medicine (2005) 11:538-544.
67) Peretto, P., et al., J. Comparative Neurology (2005) 487:407-427.
68) Scheffler, B., et al
Capsazepine: a competitive antagonist of the sensory
neurone excitant capsaicin Br J Pharmacol 107(2), 544-552
Caterina, M. J., Schumacher, M. A., Tominaga, M., Rosen, T. A., Levine, J. D.,
and Julius
potential
configurations of single guinea-pig atrial and ventricular myocytes J Physiol 368,
525-544
Ishihara, K., Yan, D. H., Yamamoto, S. and Ehara, T. (2002) Inward rectifier K(+)
current under
enzyme:substrate ratio can be changed to 1:1 and
the digestion extended to 40 hours. This may be
helpful with sample proteins that are difficult to
cleave.1
2. Incubate the reaction at 37 °C for
readings should be between 0.1–0.5. Therefore, an
volume of the plasmid DNA should be diluted from 1:40 to 1:200 with deionized water before
measuring UV absorbance. To obtain the concentration of plasmid
ml 1500 ml
Resuspension Buffer (1) 16 ml 24 m 32 ml 40 m 48 ml
Lysis Buffer (2) 16 ml 24 m 32 ml 40 m 48 ml
Mobius Neutralization Buffer (3) 16 ml 24 ml 32 ml 40
REFERENCES:
1. Cole, S.T. et al. (1998). Deciphering the biology of Mycobacterium tuberculosis from the
complete genome sequence. Nature 393:537-544.
2. Valway, S.E. et al. (
REFERENCES:
1. Cole, S.T. et al. (1998). Deciphering the biology of Mycobacterium tuberculosis from the
complete genome sequence. Nature 393:537-544.
2. Valway, S.E. et al. (
Anti-PAR-3 H M Mo Ca WB ICC Pur Rb IgG 200 mg 07-330
Protein
PAR-1Bα, active KA 10 mg 14-544
siRNA
siRNA plasmid, pKD-Par-1B-v3 H RNAi 5 mg 62-233
siRNA plasmid, pKD-Par-1B-v4 H RNAi
,NP_001119585.1 , NP_001119586.1 y y n/d
543 p53 BP1 B4561 7158 TP53BP1 P NP_001135451.1 y n/d n/d
544 p53 BP1 B4436 7158 TP53BP1 P NP_001135451.1 y n/d n/d
43 ASPP2 A4480 7159 TP53BP2 M NP_001026855.2
,NP_001119585.1 , NP_001119586.1 y y n/d
543 p53 BP1 B4561 7158 TP53BP1 P NP_001135451.1 y n/d n/d
544 p53 BP1 B4436 7158 TP53BP1 P NP_001135451.1 y n/d n/d
43 ASPP2 A4480 7159 TP53BP2 M NP_001026855.2
,NP_001119585.1 , NP_001119586.1 y y n/d
543 p53 BP1 B4561 7158 TP53BP1 P NP_001135451.1 y n/d n/d
544 p53 BP1 B4436 7158 TP53BP1 P NP_001135451.1 y n/d n/d
43 ASPP2 A4480 7159 TP53BP2 M NP_001026855.2