Escherichia coli
ATCC 8739 • WDCM 00012
/ 55’ at 20-25°C +/- 30% of the number of colonies counted at T0
Listeria monocytogenes
ATCC 13932 • WDCM 00021
/ 55’ at 20-25°C +/- 30%
Coste, B., Na+ channel Nav1.9: in
search of a gating mechanism, Trends Neurosci.,
26, 55-57 (2003).
MCT,PHC 10/5-1
Sigma brand products are sold through Sigma-Aldrich, Inc.
Sigma-Aldrich
including Src,
Fer and Syk.2-5 It represents a highly conserved family
of perimembrane signaling proteins, including the
hematopoietic lineage cell specific proteins
HS1/LCKBP1 and HIP-55.
Cortactin exists
the bottom of the tube.
Incubate 30 minutes at +55°C
Inactivate Transcriptor High Fidelity Reverse Transcriptase by heating to +85°C for 5 minutes.
Place the tube on ice.
106 cells per tube.
2. Add 5 µl of monoclonal antibody to tube(s)
containing cells to be stained. Tap tube gently to
mix. Incubate the cells at room temperature (18-
22 °C) for 30 minutes.
with the Lysis Solution for Blood at room temperature for 5 minutes for whole
blood, at 55 °C for 15 minutes for blood cards, or at 75 °C for 5–10 minutes for cell monolayers. After adding the
Neutralization
with the Lysis Solution for Blood at room temperature for 5 minutes for whole
blood, at 55 °C for 15 minutes for blood cards, or at 75 °C for 5–10 minutes for cell monolayers. After adding the
Neutralization
Na
+
channel Nav1.9: in
search of a gating mechanism, Trends Neurosci.,
26, 55-57 (2003).
TT,MCT,PHC,MAM 12/18-1
2018 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA-ALDRICH is a
anchored-oligo(dT)18 primer. The
latter is designed to bind at the beginning of the poly(A) tail
in order to generate full-length cDNAs and to prevent prim-
ing from internal sites of the poly(A) tail. The 5´-ends
concentration
of 0.1-0.2 µg/ml is recommended. The detection limit
for recombinant human FGF-18 is approximately
5 ng/lane under non-reducing and reducing conditions.
For ELISAs, a working antibody
Radius
(cm)
rpm at 300
5 g
rpm at
6,500 5 g
rpm at
12,000 5 g
rpm at
16,000 5 g
Eppendorf
5410 – 12 5.8 2,143 10,012 13,555 15,652
5415C F45-18-11 18 7.3