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Bmax value
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(Optional) DNA Polymerase for use with WGA1 (Sigma, W3891 or WGA3)
4. 96-well PCR plates, with full skirt (Sigma, P4616)
5. 96-well PCR plates, with half skirt (ABgene, AB-1100)
6. Thermowell sealing
at position P6
5. Column Preparation Solution
To process 96 samples, add 70 ml of the solution to the 96 well reservoir located at position P7.
6. Wash Solution 1
To process 96 samples, add 70 ml
interest
5. (Optional) GenElute Plant Genomic DNA Miniprep Kit (Sigma, G2N10, G2N70, and G2N250) for
use as genomic DNA control.
6. Water, molecular biology reagent (Sigma, W4502)
7. 96-well PCR
medium in size)
4. Primers for genes of interest
5. Molecular biology grade water (Sigma, W4502)
6. 96-well PCR plates, with full skirt (Sigma, P4616)
7. 96-well PCR plates, with half skirt (ABgene, AB-1100
medium in size)
4. Primers for genes of interest
5. Molecular biology grade water (Sigma, W4502)
6. 96-well PCR plates, with full skirt (Sigma, P4616)
7. 96-well PCR plates, with half skirt (ABgene, AB-1100
medium in size)
4. Primers for genes of interest
5. Molecular biology grade water (Sigma, W4502)
6. 96-well PCR plates, with full skirt (Sigma, P4616)
7. 96-well PCR plates, with half skirt (ABgene, AB-1100
, and the buffer was
removed with a pipette. The beads (62 µL settled beads)
were resuspended in 588 µL BugBuster® Master Mix (Cat.
No. 71456-4) and 105 µL of the resulting suspension
was added to each
Acad Sci
USA, 25, 588-592 (1998).
4. Lin, Z.and Floros, J. Protocol for genomic DNA preparation from fresh or
frozen serum for PCR amplification. BioTechniques, 29, 460-466 (2000).
5. Panaccio, M, et
10314-10319 (2001).
3. Mandelkow, E., Alzheimer's disease. The tangled
tale of tau. Nature, 402, 588-589 (1999).
4. Sontag, E., et al., Molecular interactions among
protein phosphatase
10314-10319 (2001).
3. Mandelkow, E., Alzheimer's disease. The tangled
tale of tau. Nature, 402, 588-589 (1999).
4. Sontag, E., et al. Molecular interactions among
protein phosphatase 2A, tau, and