using ImageJ, version 1.4.3.67.
Bright Field
24
h
GFP
48
h
72
h
Merge
SEAP expression was determined at 24, 48 and 72 h
post-transfection by removing 50 µL of the
®
BPW on its stability of productivity and sterility.
Inoculation was carried out at 0, 3, 6, 9, 24, 48, and
72 hours after media preparation. Using sterile water
(by autoclaving), prepared Readybag
its stability of productivity, selectivity and sterility.
Inoculation was carried out at 0, 3, 6, 9, 24, 48, 72 and
96 hours after media preparation. Using sterile water
(autoclaved), prepared Readybag
at 35 °C
48 hr at 35 °C &
72 hr at 25 °C
Conditions: 53% relative humidity; 48 hr incubation at 35 °C versus 48 hr incubation at
35 °C followed by 72 hr incubation at 25 °C.
C for not less
than 72 hours. According to the FDA Aseptic Guide the plates for determination of the total aerobic
bacterial count should be incubated at 30 to 35 °C for 48 to 72 hours, while the plates
C for not less than
72 hours. According to the FDA Aseptic Guide the plates for determination of the total aerobic bacterial
count should be incubated at 30 to 35 °C for 48 to 72 hours, while the plates
test
Fluorescent
count
Re-incubation
count
Traditional
count
Incubation time 48 hours 72 hours 72 hours
Average of count 47.7 44 29.7
Recovery versus
traditional
161 % Not
applicable
C for not less than
72 hours. According to the FDA Aseptic Guide the plates for determination of the total aerobic bacterial
count should be incubated at 30 to 35 °C for 48 to 72 hours, while the plates
C for not less than
72 hours. According to the FDA Aseptic Guide the plates for determination of the total aerobic bacterial
count should be incubated at 30 to 35 °C for 48 to 72 hours, while the plates
brasiliensis (SDA, 30°C) 16 h 48 h
Lactobacillus acidophilus (MRS, 37°C) 48 h 72 h
Saccharomyces cerevisiae (orange serum, 30°C) 16 h 48 h
Zygosaccharomyces bailii (ZBA, 30°C) 40 h 72 h
Candida parapsilosis
temperature.
Invert the plates and incubate one set at 34-38 °C for 40-48 h. Incubate the other set at
20-24 °C for 64-72 h.
Examine the plates as soon as they are removed from the
are added to 1 ml of sample and mixed well.
Samples are incubated at 34-38 °C for 40-48 h and 20-24 °C for 64-72 h in parallel.
The colonies per plate are counted and the microbial count
at 30 ± 1
°C for 72 h ± 3 h or acc. to ISO 17410 at 6,5 ± 1 °C for 10 days.
Acc. to APHA (Test on Proteolytic Microorganisms – Skim milk agar method) at
C for not less than
72 hours. According to the FDA Aseptic Guide the plates for determination of the total aerobic bacterial
count should be incubated at 30 to 35 °C for 48 to 72 hours, while the plates
C for not less than
72 hours. According to the FDA Aseptic Guide the plates for determination of the total aerobic bacterial
count should be incubated at 30 to 35 °C for 48 to 72 hours, while the plates
are added to 1 ml of sample and mixed well.
Samples are incubated at 34-38 °C for 40-48 h and 20-24 °C for 64-72 h in parallel.
The colonies per plate are counted and the microbial