the respective
bulk material before and after storage at ambient (25 °C / 60 % rH) and accelerated conditions
(40 °C / 75 % rH). At specified time points the bottles were inverted and the material was
generation chromogenic
substrate reaction solution (5 mM)
2. Incubate at 37 °C.
3. Add 60 µl of Innovin (supplied by Siemans) in
250 mM CaCl2 or 60 µl of Dapttin (available from
Technoclone) in
Number: 11024-24-1
Specific Rotation: -47 TO -49º (10 g/100 ml 75%
Acetic Acid at 25 °C)1
The aggregation number for digitonin is 60.
Commercial digitonin is a mixture consisting of about
five glycosides
and mouse importin (60 kDa). The antibody can
be used in ELISA, immunoblotting, immunoprecipitation,
and immunocytochemistry. The antibody recognizes
mouse importin 1, 3, 5, and 7.
The importin
three different
conditions: cold (2 – 4 °C), room temperature
(25 °C, 60 % relative humidity (rH)), and
accelerated (40 °C, 75 % rH). At each time
point, the extrudates were measured using
DSC (to
Blue Albumin and IgG Depletion Medium is
supplied as a slurry in 25% (v/v) ethanol with 75 mM
NaCl containing 60% packed medium. The medium in
the bottle must be completely resuspended prior to
removing
10 min at 70°C.
2. 5-50 μL applied to Minigel lane (0.75-1.5 mm width) and run at standard conditions. (60 mA for 2 1.5 mm Minigel
gels, 1.4 h). It is suggested that you run 5-15% acrylamide (37.5:1
solutions (at 5 mg/L intervals) spanning the 5-40
mg/L concentration range.
10.0 Calibration and Standardization
Spectroquant ® NOVA 60 Filter Photometer
The Spectroquant® NOVA 60 instrument is