doubling time; Viabilty>80% over
at least 2 passages
10:90 3.0 Cell density, viability in mid-log
growth phase
Normal cell doubling time; Viabilty>80% over
at least 2 passages
0:100 3.0 Cell
doubling time; Viability >80% over at
least 2 passages
10:90 3.0 Cell density, viability in mid-log
growth phase
Normal cell doubling time; Viability >80% over at
least 2 passages
0:100 3.0 Cell
this protocol, 80 – 90% confluence of NIH3T3 in one 10-cm plate can provide approximately 80 – 90 collagen beds
on inserts. Because NIH3T3 cells grow faster than PHKs, they can be thawed 2-3 days after
Storage 2-8°C; After opening, transfer contents to
small vial provided for your convenience.
Mattson Galaxy 3020 FT-IR
70 eV Electron Ionization Mass Spectrum
30 40 50 60 70 80 90 100 110 120
Results were normalized
using cyclophilin quantitative RT-PCR results.
0
10
20
30
40
50
60
70
80
90
100
p
LK
O
c
o
n
tr
o
l
JA
K
1
sh
R
N
A
p
LK
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NovAseptic® Valve
Valve Body, Tank Outlet Valve 180˚, Elbow 90˚ TC
Catalogue Number NU###/451-312
Valve Body, Tank Outlet Valve 180˚, Elbow 90˚ TC Configuration
Specification Sheet
D
A
Tu
kDa, ∼70 kDa, and 80–105 kDa. A
member of the latter group, HSP 90, is found in many
mammalian tissues and can constitute up to 1–2% of
the total cytosolic protein. Mammalian HSP 90 has an
affinity
Figure 2. Inactivation of the hKv2.1/hKv9.2 current.
Cells were stepped from a holding potential of –80 mV to a test pulse of 30 mV for 200 ms
following pre-pulse voltages ranging from -90 to +20 mV
–
2. Add 90 L of the appropriate Reaction Mix to each
of the wells. Mix well using a horizontal shaker or
by pipetting. Incubate the plate at plate for
10–15 minutes at 25 C or