0.005 0.0053 106
0.0125 0.0102 82
0.025 0.0236 94
Linearity
Two different patient samples were diluted with the “0”
calibrator to 1:2, 1:4 and 1:8. CRP values were assayed
and results were corrected
cycling protocol please see
Technical Guide.
Initial denaturation 94 °C 2 min
40
c
yc
le
s
Denaturation 94 °C 15 sec
Annealing
60 °C or 5 °C
below lowest
Simmons, W.H.,
Biochemistry, 30, 4997-5007 (1991).
10. Eto, I. And Bandy, M.D., Mol. Cell Biochem., 94,
19-36 (1990).
11. Iwata, M., Adachi, M. and Ishizaka, K., J.
Immunol.,140, 2534-2542 (1988
S., et al., p53DINP1, a p53-inducible
gene, regulates p53-dependent apoptosis. Mol.
Cell., 8, 85-94 (2001).
2. Tomasini, R., et al., Molecular and functional
characterization of the stress-induced
classical collagenases (MMP-1,
MMP-8, and MMP-13).
Xenopus collagenase may facilitate larval tissue
degeneration and adult organogenesis during
amphibian metamorphosis.8 MMP-18 (Xenopus
collagenase) is regulated
classical collagenases (MMP-1,
MMP-8, and MMP-13).
Xenopus collagenase may facilitate larval tissue
degeneration and adult organogenesis during
amphibian metamorphosis.8 MMP-18 (Xenopus
collagenase) is
collagenases (MMP-1,
MMP-8, and MMP-13).
Xenopus collagenase may facilitate larval tissue
degeneration and adult organogenesis during
amphibian metamorphosis.8 MMP-18 (Xenopus
collagenase) is
Anti-Collagen Type IV antibody, Mouse monoclonal
clone col-94, purified from hybridoma cell culture
Product Number SAB4200709
Product Description
Anti-Collagen Type IV antibody, Mouse monoclonal
step cycling protocol please see
Technical Guide.
Initial denaturation 94 °C 2 min
40
c
yc
le
s Denaturation 94 °C 15 seconds
Annealing 60 °C or 5 °C below lowest primer
step cycling protocol please see
Technical Guide.
Initial denaturation 94 °C 2 min
40
c
yc
le
s Denaturation 94 °C 15 sec
Annealing 60 °C or 5 °C below lowest primer
step cycling protocol please see
Technical Guide.
Initial denaturation 94 °C 2 min
40
c
yc
le
s Denaturation 94 °C 15 sec
Annealing 60 °C or 5 °C below lowest primer
biology, DNA grade, Medium
Catalog Number S6147
Store at Room Temperature
CAS Number: 9050-94-6
Product Description
Sephadex G-100, Medium is a gel filtration
chromatography product for desalting
04-18-02/MAB1120/ADN
REFERENCES: 1. Soos MA, et al. Journal of Biological Chemistry, 1992, 267(18):12955-63.
2. Schumacher R, et al. Journal of Biological Chemistry, 1993, 268(2):1087-
94.
active m-Calpain and does not
cross-react with other calpain family members, e.g.,
µ-calpain, calpain-94, ncl-2, ncl-3, etc. The antibody
reacts with bands at 80 kDa and 58 kDa, and a series
of further
).
• No reactivity shown to the following H3N2 strains:
A/Wuhan/396/95; A/Johannesburg/33/94; A/Shandung/93;A/Shanghai/16/89 and
all H3N2 strains isolated in CA 1986 to present.
• No reactivity
methylamine)
0.1 M
0.5 M
1.0 M
102
90
82
Guanidine hydrochloride 0.1 M
0.5 M
1.0 M
94
98
94
Acetonitrile 1% (v/v)
5% (v/v)
10% (v/v)
110
151
118
Safety Information
Quantitative PCR can be
stored at 2-8 °C for up to 6 months so there is no
waiting for the reaction components to thaw. It can
also be stored at –20 °C for up to 18 months.
Store the Reference
reagents and samples to room temperature (18–25 °C) before use. It is recommended that all
standards and samples be run at least in duplicate.
2. Label removable 8-well strips as appropriate for your experiment
and LP-85 and does not
cross-react with other calpain family members
(µ-calpain, m-calpain, calpain-94, ncl-2, ncl-3, etc). By
immunoblotting against the reduced protein, the
antibody reacts with bands