2003) 6:103-111.
RELATED
REFERENCES:
Sakurai, T et al. (1998) Cell. 92:573-585.
DeLecca, L et al. (1998) PNAS. 95:322-327.
Important Note:
During shipment, small volumes of product
imidazole,
0.1 mM PMSF, 0.25 mM DTT, and 25% glycerol.
Molecular mass: ∼92 kDa
Purity: 70–95% (SDS-PAGE, see Figure 1)
Precautions and Disclaimer
This product is for R&D use only, not for drug
mM EDTA,
0.25 mM DTT, 0.1 mM PMSF, and and 25% glycerol.
Molecular mass: ∼92 kDa
Purity: 70–95% (SDS-PAGE, see Figure 1)
Precautions and Disclaimer
This product is for R&D use only, not for drug
Kessler, C., et al., Gene, 92, 1 1990.
8/99
Sigma brand products are sold through Sigma-Aldrich, Inc.
Sigma-Aldrich, Inc. warrants that its products conform to the information contained in this and other
.1 mM PMSF, and 25%
glycerol.
Molecular mass: ∼110 kDa
Purity: 70–95% (SDS-PAGE, see Figure 1)
Specific Activity: 68–92 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This product
recommend
determining the optimal working dilutions by titration.
References
1. Barroso, M., et al., Proc. Natl. Acad. Sci. USA, 92,
527-531 (1995).
2. Nelson, D.S., et al., J. Cell Biol., 143, 319-331
at least 10 µg/ml using A431
cells fixed with 95% ethanol and 5% acetic acid.
By immunoprecipitation, 4 µg will immunoprecipitate
STAT3 from 0.5-1 mg of an EGF stimulated human
A431 cell lysate.
repeated
freeze/thaw cycles.
RELATED
REFERENCES:
1. Sakurai, T et al. (1998) Cell. 92:573-585.
2. DeLecca, L et al. (1998) PNAS. 95:322-327.
Important Note: During shipment, small volumes