96924

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1,1,1,3,3,3-Hexafluoro-2-propanol and 2,2,2-trifluoroethanol solvents induce self-assembly with different surface morphology in an aromatic dipeptide.

Samala Murali Mohan Reddy et al.

Organic & biomolecular chemistry, 12(32), 6181-6189 (2014-07-08)

Peptide based self-assembled structures, especially those from smaller peptides, have attracted much research interest due to their potential applications as biomaterials. These structures have been produced using different solvents (one of the methods), including alcohols, except fluorinated alcohols, which are

Multi-omic Profiling Reveals Dynamics of the Phased Progression of Pluripotency.

Pengyi Yang et al.

Cell systems, 8(5), 427-445 (2019-05-13)

Pluripotency is highly dynamic and progresses through a continuum of pluripotent stem cell states. The two states that bookend the pluripotency continuum, naive and primed, are well characterized, but our understanding of the intermediate states and transitions between them remains

The role of conformational flexibility in β2-microglobulin amyloid fibril formation at neutral pH.

John P Hodkinson et al.

Rapid communications in mass spectrometry : RCM, 26(16), 1783-1792 (2012-07-11)

Amyloid formation is implicated in a number of human diseases. β(2)-Microglobulin (β(2)m) is the precursor protein in dialysis-related amyloidosis and it has been shown that partial, or more complete, unfolding is key to amyloid fibril formation in this pathology. Here

Conversion of natively unstructured α-synuclein to its α-helical conformation significantly attenuates production of reactive oxygen species.

Binbin Zhou et al.

Journal of inorganic biochemistry, 118, 68-73 (2012-11-06)

The intracellular α-synuclein (α-syn) protein, whose conformational change and aggregation have been closely linked to the pathology of Parkingson's disease (PD), is highly populated at the presynaptic termini and remains there in the α-helical conformation. In this study, circular dichroism

Subunit compositions of the RNA-silencing enzymes Pol IV and Pol V reveal their origins as specialized forms of RNA polymerase II.

Thomas S Ream et al.

Molecular cell, 33(2), 192-203 (2008-12-27)

In addition to RNA polymerases I, II, and III, the essential RNA polymerases present in all eukaryotes, plants have two additional nuclear RNA polymerases, abbreviated as Pol IV and Pol V, that play nonredundant roles in siRNA-directed DNA methylation and

Influence of bone marrow-derived mesenchymal stem cells pre-implantation differentiation approach on periodontal regeneration in vivo.

Xinjie Cai et al.

Journal of clinical periodontology, 42(4), 380-389 (2015-02-19)

The implantation of bone marrow-derived mesenchymal stem cells (MSCs) has previously been shown successful to achieve periodontal regeneration. However, the preferred pre-implantation differentiation strategy (e.g. maintenance of stemness, osteogenic or chondrogenic induction) to obtain optimal periodontal regeneration is still unknown.

Chiral Zn(II)-bisamidine complex as a Lewis-Brønsted combined acid catalyst: application to asymmetric Mukaiyama aldol reactions of α-ketoesters.

Ryo Gotoh et al.

Molecules (Basel, Switzerland), 17(8), 9010-9022 (2012-08-01)

Focusing on the steric and electronic properties of the resonance-stabilized amidine framework, a cationic metal-bisamidine complex was designed as a conjugated combined Lewis-Brønsted acid catalyst. The chiral Zn(II)-bisamidine catalyst prepared from the 2,2'-bipyridyl derived bisamidine ligand, ZnCl₂, and AgSbF₆ promoted

The many roles for fluorine in medicinal chemistry.

William K Hagmann

Journal of medicinal chemistry, 51(15), 4359-4369 (2008-06-24)

Truncation of a β-barrel scaffold dissociates intrinsic stability from its propensity to aggregation.

Lucrecia M Curto et al.

Biophysical journal, 103(9), 1929-1939 (2012-12-04)

Δ98Δ is a functional all-β sheet variant of intestinal fatty acid binding protein (IFABP) that was generated by controlled proteolysis. This framework is useful to study the molecular determinants related to aggregation of β-barrel proteins. Albeit displaying increased conformational plasticity

Trifluoroethanol-induced stabilization of the alpha-helical structure of beta-lactoglobulin: implication for non-hierarchical protein folding.

K Shiraki et al.

Journal of molecular biology, 245(2), 180-194 (1995-01-13)

Trifluoroethanol (TFE) is known to stabilize the alpha-helical structure in proteins and their fragments. However, the relationship between the TFE-induced structures and the native structure is not clear. Here we show that beta-lactoglobulin, which consists predominantly of beta-sheets, exhibited a

The accessory helix of complexin functions by stabilizing central helix secondary structure.

Daniel T Radoff et al.

eLife, 3 (2014-11-11)

The presynaptic protein complexin (CPX) is a critical regulator of synaptic vesicle fusion, but the mechanisms underlying its regulatory effects are not well understood. Its highly conserved central helix (CH) directly binds the ternary SNARE complex and is required for

Quantification of serum IgG subclasses by use of subclass-specific tryptic peptides and liquid chromatography--tandem mass spectrometry.

Paula M Ladwig et al.

Clinical chemistry, 60(8), 1080-1088 (2014-05-07)

Measurement of IgG subclasses is a useful tool for investigation of humoral immune deficiency in the presence of total IgG within reference intervals and IgG4-related disease. Nephelometry has been the method of choice for quantification. We describe an LC-MS/MS method

Scaffold architecture controls insulinoma clustering, viability, and insulin production.

Britani N Blackstone et al.

Tissue engineering. Part A, 20(13-14), 1784-1793 (2014-01-15)

Recently, in vitro diagnostic tools have shifted focus toward personalized medicine by incorporating patient cells into traditional test beds. These cell-based platforms commonly utilize two-dimensional substrates that lack the ability to support three-dimensional cell structures seen in vivo. As monolayer

Solvent-induced tuning of internal structure in a protein amyloid protofibril.

Anjali Jha et al.

Biophysical journal, 103(4), 797-806 (2012-09-06)

An important goal in studies of protein aggregation is to obtain an understanding of the structural diversity that is characteristic of amyloid fibril and protofibril structures at the molecular level. In this study, what to our knowledge are novel assays

Toward a common aggregation mechanism for a β-barrel protein family: insights derived from a stable dimeric species.

Carla R Angelani et al.

Biochimica et biophysica acta, 1844(9), 1599-1607 (2014-06-15)

Δ78Δ is a second generation functional all-β sheet variant of IFABP (intestinal fatty acid binding protein) corresponding to the fragment 29-106 of the parent protein. This protein and its predecessor, Δ98Δ (segment 29-126 of IFABP), were initially uncovered by controlled

Substantial Differentiation of Human Neural Stem Cells Into Motor Neurons on a Biomimetic Polyurea.

Donghwa Yun et al.

Macromolecular bioscience, 15(9), 1206-1211 (2015-06-03)

To find the first restorative treatment for spinal cord injury (SCI), researchers have focused on stem cell therapies. However, one obstacle is the lack of an implantable cell scaffold that can support efficient motor neuron (MN) differentiation and proliferation. We

Detection and analysis of protofibrils and fibrils of hemoglobin: implications for the pathogenesis and cure of heme loss related maladies.

Afshin Iram et al.

Archives of biochemistry and biophysics, 533(1-2), 69-78 (2013-03-19)

TFE induces structural alterations of proteins similar to the lipid environment of biological membranes, implicating these studies worthy of analyzing protein conformation in membranes such as red blood cells (RBCs). Heme loss occurs on rupturing of RBCs as found in

Atomic-resolution structures of horse liver alcohol dehydrogenase with NAD(+) and fluoroalcohols define strained Michaelis complexes.

Bryce V Plapp et al.

Biochemistry, 51(19), 4035-4048 (2012-04-26)

Structures of horse liver alcohol dehydrogenase complexed with NAD(+) and unreactive substrate analogues, 2,2,2-trifluoroethanol or 2,3,4,5,6-pentafluorobenzyl alcohol, were determined at 100 K at 1.12 or 1.14 Å resolution, providing estimates of atomic positions with overall errors of ~0.02 Å, the

Two-dimensional electrophoresis of human placental mitochondria and protein identification by mass spectrometry: toward a human mitochondrial proteome.

T Rabilloud et al.

Electrophoresis, 19(6), 1006-1014 (1998-06-25)

Owing to the complexity of higher eukaryotic cells, characterization of a complete proteome is likely to be difficult to achieve. However, advantage can be taken of the cell compartmentalization to build organelle proteomes, which can moreover be viewed as specialized

Metrological traceability in mass spectrometry-based targeted protein quantitation: a proof-of-principle study for serum apolipoproteins A-I and B100.

Nico P M Smit et al.

Journal of proteomics, 109, 143-161 (2014-06-28)

In this study, we have followed up on previous liquid chromatography (LC) multiple reaction monitoring (MRM) mass spectrometry (MS) approaches for measurement of apolipoprotein (apo) A-I and apo B100 in serum aiming for implementation of a multiplexed assay in a

Optical and electron paramagnetic resonance studies of the excited triplet states of UV-B absorbers: 2-ethylhexyl salicylate and homomenthyl salicylate.

Kazuto Sugiyama et al.

Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology, 14(9), 1651-1659 (2015-07-03)

The energy levels and lifetimes of the lowest excited triplet (T1) states of UV-B absorbers, 2-ethylhexyl salicylate (EHS) and homomenthyl salicylate (HMS), and their deprotonated anions (EHS(-) and HMS(-)) were determined through measurements of phosphorescence and electron paramagnetic resonance (EPR)

Separation of racemic 1-(9-Anthryl)-2,2,2-trifluoroethanol by sub-/supercritical fluid chromatography.

Xiqin Yang et al.

Methods in molecular biology (Clifton, N.J.), 970, 249-255 (2013-01-04)

Supercritical fluid chromatography is an environmentally benign separation technology due to the use of carbon dioxide modified with organic solvents as the mobile phase. It has found widespread use in the separation of chiral compounds based on the relative ease

Cell-penetrating antimicrobial peptides - prospectives for targeting intracellular infections.

Jesper S Bahnsen et al.

Pharmaceutical research, 32(5), 1546-1556 (2015-03-18)

To investigate the suitability of three antimicrobial peptides (AMPs) as cell-penetrating antimicrobial peptides. Cellular uptake of three AMPs (PK-12-KKP, SA-3 and TPk) and a cell-penetrating peptide (penetratin), all 5(6)-carboxytetramethylrhodamine-labeled, were tested in HeLa WT cells and analyzed by flow cytometry

Measuring the relative hydrogen-bonding strengths of alcohols in aprotic organic solvents.

Malcolm E Tessensohn et al.

Chemphyschem : a European journal of chemical physics and physical chemistry, 16(1), 160-168 (2014-11-25)

Voltammetric experiments with 9,10-anthraquinone and 1,4-benzoquinone performed under controlled moisture conditions indicate that the hydrogen-bond strengths of alcohols in aprotic organic solvents can be differentiated by the electrochemical parameter ΔEp (red) =|Ep (red(1)) -Ep (red(2)) |, which is the potential

Intrinsic disorder in Viral Proteins Genome-Linked: experimental and predictive analyses.

Eugénie Hébrard et al.

Virology journal, 6, 23-23 (2009-02-18)

VPgs are viral proteins linked to the 5' end of some viral genomes. Interactions between several VPgs and eukaryotic translation initiation factors eIF4Es are critical for plant infection. However, VPgs are not restricted to phytoviruses, being also involved in genome

Trifluoroethanol and acetonitrile induced formation of the molten globule states and aggregates of cellulase.

Afshin Iram et al.

International journal of biological macromolecules, 50(4), 932-938 (2012-06-09)

A systematic investigation on the effects of trifluoroethanol and acetonitrile at various concentrations on cellulase (EC 3.2.1.4) was studied by enzyme assay, intrinsic fluorescence, ANS binding, circular dichroism and ATR-Fourier transform infra red spectroscopy. The results show the presence of

Visualizing the Inner Architecture of Poly(ϵ-caprolactone)-Based Biomaterials and Its Impact on Performance Optimization.

Adam J P Bauer et al.

Macromolecular bioscience, 15(11), 1554-1562 (2015-07-15)

The performance of poly(ϵ-caprolactone) (PCL)-based biomaterials is defined by spatial distributions of PCL's amorphous and crystalline domains. Unfortunately, directly visualizing their inner architectures has been challenging. This study demonstrates, the superior degradation selectivity of Candida antarctica lipase B (CALB) enzyme;

The structure and mechanical properties of the proteins of lamprey cartilage.

Ellen M Green et al.

Biopolymers, 103(4), 187-202 (2014-11-05)

The cyanogen bromide-resistant proteins of lamprey cartilage are biochemically related to the mammalian elastic protein, elastin. This study investigates their mechanical properties and enquires whether, like elastin, long-range elasticity arises in them from a combination of entropic and hydrophobic mechanisms.

Trifluoroethanol-induced unfolding of concanavalin A: equilibrium and time-resolved optical spectroscopic studies.

Qi Xu et al.

Biochemistry, 44(22), 7976-7987 (2005-06-01)

Conformational structure changes in concanavalin A (Con A), a legume lectin protein which is composed of 18 beta-strands, induced by dissolving in 50% trifluoroethanol (TFE) were monitored at neutral and low pH by far- and near-UV circular dichroism (CD), fluorescence

Remarkable effect of CF3CH2OH for the halogen induced oxidative rearrangement reaction of aminals leading to 3,4-dihydroquinazolines.

Kenichi Murai et al.

Organic & biomolecular chemistry, 11(16), 2648-2651 (2013-03-06)

CF3CH2OH was found to be a useful solvent for the oxidative rearrangement reactions of aminals promoted by N-chlorosuccinimide, which proceed via the intermediacy of in situ formed chloro-aminals and that produce 3,4-dihydroquinazolines.

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