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关键词:'A3377'
显示 1-30 共 41 条结果 关于 "A3377" 范围 论文
Butaek Lim et al.
Sensors (Basel, Switzerland), 15(8), 17977-17989 (2015-07-28)
We report a simple detection of protein kinase activity using Zn(II)-mediated fluorescent resonance energy transfer (FRET) between quantum dots (QDs) and dye-tethered peptides. With neither complex chemical ligands nor surface modification of QDs, Zn(II) was the only metal ion that
Allosteric modulation of ATP-gated P2X receptor channels.
Coddou, C., et al.
Rev. Neuro., 22, 335-354 (2011)
Perturbation of phosphoglycerate kinase 1 (PGK1) only marginally affects glycolysis in cancer cells.
Chengmeng Jin et al.
The Journal of biological chemistry, 295(19), 6425-6446 (2020-03-29)
Phosphoglycerate kinase 1 (PGK1) plays important roles in glycolysis, yet its forward reaction kinetics are unknown, and its role especially in regulating cancer cell glycolysis is unclear. Here, we developed an enzyme assay to measure the kinetic parameters of the
Fabian Sesterhenn et al.
PLoS biology, 17(2), e3000164-e3000164 (2019-02-23)
Throughout the last several decades, vaccination has been key to prevent and eradicate infectious diseases. However, many pathogens (e.g., respiratory syncytial virus [RSV], influenza, dengue, and others) have resisted vaccine development efforts, largely because of the failure to induce potent
Christopher J Groten et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 35(6), 2747-2765 (2015-02-13)
It is unknown whether neurons can dynamically control the capacity for secretion by promptly changing the number of plasma membrane voltage-gated Ca(2+) channels. To address this, we studied peptide release from the bag cell neurons of Aplysia californica, which initiate
Teresa Obis et al.
Molecular brain, 8(1), 80-80 (2015-12-03)
Various protein kinase C (PKC) isoforms contribute to the phosphorylating activity that modulates neurotransmitter release. In previous studies we showed that nPKCε is confined in the presynaptic site of the neuromuscular junction and its presynaptic function is activity-dependent. Furthermore, nPKCε
Yuan Liu et al.
Carbohydrate research, 401, 1-4 (2014-12-03)
Three sugar 1-phosphates that are donor substrates for phosphorylases were produced at the gram scale from phosphoenolpyruvic acid and the corresponding sugars by the combined action of pyruvate kinase and the corresponding anomeric kinases in good yields. These sugar 1-phosphates
Sarah Triclin et al.
Nature materials, 20(6), 883-891 (2021-01-23)
Microtubule instability stems from the low energy of tubulin dimer interactions, which sets the growing polymer close to its disassembly conditions. Molecular motors use ATP hydrolysis to produce mechanical work and move on microtubules. This raises the possibility that the
Aurélie Formey et al.
Cell and tissue research, 343(3), 509-519 (2011-01-19)
Cells with irregular shapes, numerous long thin filaments, and morphological similarities to the gastrointestinal interstitial cells of Cajal (ICCs) have been observed in the wall of some blood vessels. These ICC-like cells (ICC-LCs) do not correspond to the other cell
Angélica T Vieira et al.
Arthritis & rheumatology (Hoboken, N.J.), 67(6), 1646-1656 (2015-04-29)
Host-microbial interactions are central in health and disease. Monosodium urate monohydrate (MSU) crystals cause gout by activating the NLRP3 inflammasome, leading to interleukin-1β (IL-1β) production and neutrophil recruitment. This study was undertaken to investigate the relevance of gut microbiota, acetate
Monica Frinchi et al.
International journal of molecular sciences, 21(23) (2020-12-10)
Acute or chronic administration of guanosine (GUO) induces anxiolytic-like effects, for which the adenosine (ADO) system involvement has been postulated yet without a direct experimental evidence. Thus, we aimed to investigate whether adenosine receptors (ARs) are involved in the GUO-mediated
Crosstalk between purinergic receptors and canonical signaling pathways in the mouse salivary gland.
Sumit Bhattacharya et al.
Cell calcium, 58(6), 589-597 (2015-10-08)
Isolated clusters of mouse parotid acinar cells in combination with live cell imaging were used to explore the crosstalk in molecular signaling between purinergic, cholinergic and adrenergic pathways that integrate to control fluid and protein secretion. This crosstalk was manifested
J E Geiger et al.
Neuroscience, 162(4), 1023-1038 (2009-05-12)
Ca(2+) influx through voltage-gated Ca(2+) channels is a fundamental signaling event in neurons; however, non-traditional routes, such as non-selective cation channels, also permit Ca(2+) entry. The present study examines the Ca(2+) permeability of a cation channel that drives an afterdischarge
Tânia D F Costa et al.
Nature communications, 10(1), 3589-3589 (2019-08-11)
Overcoming cellular growth restriction, including the evasion of cellular senescence, is a hallmark of cancer. We report that PAK4 is overexpressed in all human breast cancer subtypes and associated with poor patient outcome. In mice, MMTV-PAK4 overexpression promotes spontaneous mammary
W Liu et al.
Life sciences, 89(5-6), 141-146 (2011-06-21)
Microglia and astrocytes in the central nervous system are now recognized as active participants in various pathological conditions such as trauma, stroke, or chronic neurodegenerative disorders. Their activation is closely related with the development and severity of diseases. Interestingly, activation
Hong-Mei Guo et al.
Proceedings of the National Academy of Sciences of the United States of America, 112(14), 4435-4440 (2015-04-02)
The airway epithelia initiate and modulate the inflammatory responses to various pathogens. The cystic fibrosis transmembrane conductance regulator-mediated Cl(-) secretion system plays a key role in mucociliary clearance of inhaled pathogens. We have explored the effects of Toxoplasma gondii, an
Scott H Randell et al.
Methods in molecular biology (Clifton, N.J.), 742, 285-310 (2011-05-07)
When primary human airway epithelial (hAE) cells are grown in vitro on porous supports at an air-liquid interface (ALI), they recapitulate in vivo morphology and key physiologic processes. These cultures are useful for studying respiratory tract biology and diseases and
Diana C Vanegas et al.
Biosensors & bioelectronics, 74, 37-44 (2015-06-22)
The objective of this study was to develop a self-referencing electrochemical biosensor for the direct measurement of ATP flux into the extracellular matrix by living cells/organisms. The working mechanism of the developed biosensor is based on the activity of glycerol
Fabiano B Carvalho et al.
The Journal of nutritional biochemistry, 26(4), 378-390 (2015-01-31)
The aim of this study was to investigate the protective effect of anthocyanins (ANT) on oxidative and inflammatory parameters, as well as ion pump activities, in the pons of rats experimentally demyelinated with ethidium bromide (EB). Rats were divided in
Rui Xiong et al.
Molecular pharmacology, 88(6), 1045-1054 (2015-09-26)
A potential cause of neurodegenerative diseases, including Parkinson's disease (PD), is protein misfolding and aggregation that in turn leads to neurotoxicity. Targeting Hsp90 is an attractive strategy to halt neurodegenerative diseases, and benzoquinone ansamycin (BQA) Hsp90 inhibitors such as geldanamycin
Lizhen Qiao et al.
Journal of chromatography. A, 1360, 240-247 (2014-08-19)
Glucaminium-based ionic liquids are a new class of recently developed ionic liquids and prepared by functionalizing the amine group of N-methyl-d-glucamine, which renders them good hydrophilicity due to the presence of the glucose structure and charged quaternary ammonium group. In
Mirkko Flecken et al.
Cell, 183(2), 457-473 (2020-09-27)
Rubisco, the key enzyme of CO2 fixation in photosynthesis, is prone to inactivation by inhibitory sugar phosphates. Inhibited Rubisco undergoes conformational repair by the hexameric AAA+ chaperone Rubisco activase (Rca) in a process that is not well understood. Here, we
Xi-Lan Tang et al.
Inflammation, 38(1), 94-101 (2014-09-06)
Inflammatory response is an important mechanism in the pathogenesis of cardiovascular diseases. Cardiac fibroblasts play a crucial role in cardiac inflammation and might become a potential therapeutic target in cardiovascular diseases. Kaempferol, a flavonoid commonly existing in many edible fruits
Weerachat Sompong et al.
PloS one, 10(6), e0129495-e0129495 (2015-06-09)
Ferulic acid (FA) is the ubiquitous phytochemical phenolic derivative of cinnamic acid. Experimental studies in diabetic models demonstrate that FA possesses multiple mechanisms of action associated with anti-hyperglycemic activity. The mechanism by which FA prevents diabetes-associated vascular damages remains unknown.
Xinran Geng et al.
Biochemical pharmacology, 182, 114226-114226 (2020-09-26)
Successful drug repurposing relies on the understanding of molecular mechanisms of the target compound. Cardiac glycosides have demonstrated potent anticancer activities; however, the pharmacological mechanisms underlying their anticancer effects remained elusive, which has restricted their further development in cancer treatment.
Peifa Yu et al.
Virology, 546, 109-121 (2020-05-27)
The inflammasome machinery has recently been recognized as an emerging pillar of innate immunity. However, little is known regarding the interaction between the classical interferon (IFN) response and inflammasome activation in response to norovirus infection. We found that murine norovirus
G Baffy et al.
British journal of cancer, 105(4), 469-474 (2011-06-30)
Recent findings link metabolic transformation of cancer cells to aberrant functions of mitochondrial uncoupling proteins (UCPs). By inducing proton leak, UCPs interfere with mitochondrial synthesis of adenosine 5'-triphosphate, which is also a key determinant of glycolytic pathways. In addition, UCP
Zachary M March et al.
eLife, 9 (2020-12-16)
The AAA+ protein disaggregase, Hsp104, increases fitness under stress by reversing stress-induced protein aggregation. Natural Hsp104 variants might exist with enhanced, selective activity against neurodegenerative disease substrates. However, natural Hsp104 variation remains largely unexplored. Here, we screened a cross-kingdom collection
Nora Grahl et al.
PLoS pathogens, 11(8), e1005133-e1005133 (2015-09-01)
Candida albicans is both a major fungal pathogen and a member of the commensal human microflora. The morphological switch from yeast to hyphal growth is associated with disease and many environmental factors are known to influence the yeast-to-hyphae switch. The
Hoseok Choi et al.
Biosensors & bioelectronics, 73, 93-99 (2015-06-09)
We report a simple method for analyzing sequential phosphorylation by protein kinases using fluorescent peptide substrates and microfluidic isoelectric focusing (μIEF) electrophoresis. When a dye-labeled peptide substrate was sequentially phosphorylated by two consecutive protein kinases (mitogen-activated protein kinase (MAPK) and
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