Low pressure liquid chromatography

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facet applications:Low pressure liquid chromatography

facet content type:Technical Article

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Purification Using Protein A-based Chromatography Media

This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.

Purification or Removal of Proteins and Peptides

This page shows how to purify or remove proteins and peptides with exposed amino acids with Chelating Sepharose High Performance, Chelating Sepharose Fast Flow, Capto Chelating from Cytiva.

Purification or Removal of Viruses including Adeno-associated Virus

This page shows how to purify or remove viruses with a Capto DeVirS, AVB Sepharose High Performance from Cytiva.

Purification using GST SpinTrap™

This page shows how to purify GST-tagged proteins using GST SpinTrap™ from Cytiva.

Performing a Purity and Homogeneity Check

This page shows how to perform a purification and homogeneity check of membrane proteins with products from Cytiva.

Troubleshooting Guide for Affinity Chromatography of Tagged Proteins

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.

UHPLC Analysis of Structurally Similar Compounds: A Case Study of Ephedrine and Related Analogs on a Carbon Column

Ultra-high performance liquid chromatographic (UHPLC) separation of ephedrine and its three structural analogs based on polar retention effect on graphite (PREG) mechanism using Supel™ Carbon LC column.

UHPLC Vitamin D Analysis on Supel™ Carbon LC Columns

UHPLC separation of Vitamin D Metabolites using Supel™ Carbon LC column ensures excellent peak shape and sensitivity.

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

Bind/Elute vs Flowthrough Mode in Multimodal Chromatography

This page describes the difference between bind/elute and ﬂowthrough modes in multimodal chromatography using Cytiva products.

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