Protein pulldown

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facet applications:Protein pulldown

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Imprint®染色质免疫沉淀试剂盒（CHP1）实验方案

Sigma Imprint染色质免疫沉淀试剂盒利用检测板系统，可实现快速的高通量ChIP检测

硫酸葡聚糖

硫酸葡聚糖以钠盐形式供应，使其在水中可溶且稳定。硫酸葡聚糖含有大约17%的硫，相当于每个葡糖基残基大约有2.3个硫酸基团。

葡聚糖

葡聚糖是葡萄糖酐聚合物。它由大约 95% 的alpha-D-(1-6)键合组成。其余的 a (1-3) 键合构成了葡聚糖的分支。分支长度的冲突数据意味着平均分支长度小于三个葡萄糖单位。但是，其他方法表明存在大于 50 个葡萄糖单位的分支。

Performing a Purification of IgG Antibodies with Protein G HP SpinTrap™/Ab SpinTrap™

This page shows how to separate IgG antibodies by afﬁnity chromatography using Protein G HP SpinTrap™/Ab SpinTrap™ from Cytiva.

Membrane Scaffold Proteins: Nanodisc Formation

Nanodisc technology aids membrane protein solubilization, overcoming associated challenges in diverse protein classes.

Dextran

Dextran polymer details: composed mainly of alpha-D-(1-6) linkages with varied branch lengths.

Antibody Purification using Protein A/G/L Agarose

Antibody purification protocols yield preparations containing endogenous IgG alongside specific antibodies.

Dextran Sulfate

Dextran sulfates as sodium salts are water-soluble and stable, containing approximately 17% sulfur for various applications.

Imprint® Chromatin Immunoprecipitation Kit (CHP1) Protocol

The Sigma Imprint Chromatin Immunoprecipitation Kit uses a plate based system to allow rapid ChIP assays in a high throughput format

Immunoprecipitation Kit (Protein G) Protocol & Troubleshooting

Immunoprecipitation Kit (Protein A) Protocol & Troubleshooting

Purification from Unclariﬁed Cell Lysate using HisTrap™ FF Crude

This page shows how to purify histidine-tagged recombinant proteins from unclariﬁed cell lysate using HisTrap FF crude from Cytiva.

Performing a Purification of IgG Antibodies with MabSelect Sure

This page shows how to separate IgG antibodies by afﬁnity chromatography using MabSelect Sure from Cytiva.

Sera-Mag™ SpeedBeads Protocol

Sera-Mag SpeedBeads provide fast and convenient protein isolation; suitable for manual or automated magnetic affinity binding in various applications.

Separation with Cytiva Products (GST Fusion Proteins)

Affinity chromatography tags like GST fusion proteins facilitate separations in various purification products from Cytiva.

Immunoprecipitation Procedure

Techniques for protein antigen molecular weight determination, protein interactions, enzymatic activity, and post-translational modifications.