mouse embryonic fibroblasts

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关键词:'mouse embryonic fibroblasts'

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Automated Differentiation of Stem Cells with the Freedom EVO and Millicell-24 Filter Plates

ESCs are usually co-cultured with mouse embryonic fibroblasts during expansion to maintain the ESCs in an undifferentiated state; removal of the fibroblasts is necessary to initiate differentiation

FibroGRO™ Media Products for culture of Human Fibroblasts

formulations provide an optimal cell culture environment for many types of human fibroblasts and mouse embryonic fibroblasts These low serum/serum-free media formulations have been shown to grow these cells

FibroGRO™ Media Products for culture of Human Fibroblasts

HESCA-1 and HESCA-2: Novel Antibodies for the Identification of Human Embryonic Stem Cells

BG01v, BG01, and BG02 are hESC cell lines; MEF: murine embryonic fibroblasts; Hs-27: human foreskin fibroblasts; mESC: murine embryonic stem cells. Figure 1. HESCA-1 and HESCA-2 stain hESCs

Accumax Dissociation Reagent for the Serial Passaging of Human Embryonic Stem Cells

hESC culture H9 human ES cells were maintained on CF1 mouse embryonic fibroblasts (Cat. No. PMEF-CF) seeded at a density of 40,000 fibroblasts/cm2 on tissue culture plates coated with growth

Millipore Tools for Characterizing Induced Pluripotent Stem Cells

adult human fibroblasts by defined factors. Cell 131: 1-12 (2007). 5. Takahashi, K., and Yamanaka, S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined

RESGRO¿ Culture Medium 500 mL

mitotically inactivated mouse embryonic fibroblast cells. 2. After 2 passages, replate 1/3 - 1/5 of the cell suspension on the same dish without inactivated mouse embryonic fibroblast cells. 3. After

Mouse iPS Cell Boost Supplement

PMEF-CF) 3 Reprogramming Mouse Somatic Cells SECTION 1: REPROGRAMMING MOUSE EMBRYONIC FIBROBLAST S Important note 1: The Mouse iPS Cell Boost Supplement

Mouse STEMCCA Dox-Inducible Polycistronic (OKSM) Lentivirus Reprogramming Kit

of mouse iPS cells from mouse embryonic fibroblasts (MEFs). Mouse iPS cells display characteristic ES cell-like morphology, stained positive for alkaline phosphatase, expressed the correct mouse ES cell

Poster: Expansion of Human Embryonic Stem Cells by Membrane Based Co-Culture

interfere with these processes. H9 Human embryonic stem cells were cultured in DMEM/F-12 media supplemented with bFGF either by direct co-culture on a mouse embryonic feeder layer in Matrigel® coated (1:30

STEMCCA Constitutive Polycistronic (OKSM) Lentivirus Reprogramming Kit

validated for the generation of mouse and human induced pluripotent stem cells from mouse embryonic fibroblasts (MEFs) and human foreskin fibroblasts (HFFs), respectively. Both mouse and human iPS cells display

HEScGRO Xeno-Free Medium for Human Embryonic Stem Cell Culture

conditions that were being used at that time to grow mouse ES cells2, including the use of feeder layers made up of mouse embryonic fibroblasts. These culture conditions were clearly not optimal

Mouse STEMCCA Cre-Excisable Constitutive Polycistronic (OKS) Lentivirus Reprogramming Kit

Reprogramming Mouse Somatic Cells SECTION 1: REPROGRAMMING MOUSE EMBRYONIC FIBROBLAST S Important note: The following protocol has been optimized using early passage primary mouse embryo fibroblasts (MEFs

Collagenase Type I for Enzymatic Passaging of Human Embryonic Stem Cells in HEScGRO Medium

1 Collagenase Type I for Enzymatic Passaging of Human Embryonic Stem Cells in HEScGRO™ Medium Abstract Human embryonic stem cells (hESCs) cultured in HEScGRO, a proprietary serum-free and

Data Sheet - S0191

important cell signaling molecule expressed during embryonic development. Shh has been shown to be involved in the patterning of the developing embryonic nervous system, somite, and limbs. Particularly

RECOMBINANT ANIMAL-FREE HUMAN FIBROBLAST GROWTH FACTOR-BASIC

/mL for the culture of human ES cells with a feeder layer, or to 8 ng/mL to supplement mouse embryonic fibroblast- conditioned medium (for feeder-free human ES cell culture).

Mouse STEMCCA Cre-Excisable Dox-Inducible Polycistronic (OKSM) Lentivirus Reprogramming Kit

of mouse iPS cells from mouse embryonic fibroblasts (MEFs). Mouse iPS cells display characteristic ES cell-like morphology, stained positive for alkaline phosphatase, expressed the correct mouse ES

Stem Cell and Specialty Cell Culture Product Guide

CEllS www.millipore.com 22 Primary Mouse Embryo Fibroblasts Many embryonic stem cell culture protocols necessitate the use of primary mouse embryo fibroblast (PMEF) cells. In these protocols, ES

Anti- p130CAS (C0354)

and migration are severely impaired in p130CAS-deficient primary fibroblasts,13,14 suggesting a crucial role of p130CAS in embryonic development, particularly in cardiovascular development and in cytoskeletal

Data Sheet - C7863

essential for mouse embryo development.10,11 In embryonic tissues or in cells derived from these embryos, Src, Fyn and Lyn kinase activities are greatly enhanced. In Csk-deficient mouse embryonic fibroblasts

Data Sheet - SAB4200760

cells. Anti-Fibronectin antibody, Mouse monoclonal specifically recognizes Fibronectin from plasma and in the pericellular extracellular matrix of cultured fibroblasts from human1-2 and mouse3 origin.

RAT ANTI-MOUSE ENDOGLIN [CD105]

macrophages and lymphoid and myeloid leukemic cells High levels of mouse endoglin mRNA have been detected in ovary, uterus, and NCTC-2071 fibroblasts, and to a lesser extent, in heart and muscle. In addition

Indirect Co-culture of Embryonic Stem Cells with Embryonic Fibroblasts

in a 500 mL format and may be used for routine mouse embryonic stem cell culture. pricing PMEF-CFL EmbryoMax Primary Mouse Embryonic Fibroblasts pricing SF008 ESGRO Complete Gelatin Solution

Reprogramming Application Note Human Non-inducible OSLN

including mouse and human. Here we show that co-transduction of the viruses from this set induces reprogramming in human foreskin fibroblast BJ cells grown on a mouse embryonic fibroblast feeder layer

Data Sheet - F1802

Recombinant Mouse Fibroblast Growth Factor 8c (FGF-8c) is measured by its ability to stimulate 3H-thymidine incorporation in a confluent quiescent culture of NR6R-3T3 fibroblasts.14, 15

Data Sheet - T3413

immunized with partially purified mouse tenascin. Monoclonal Anti-Mouse Tenascin1 reacts specifically with mouse tenascin. In supernatants of cultured mouse fibroblasts, the antibody precipitates two

Data Sheet - L5158

(LIF) from mouse, a 20 kDa protein containing 180 amino acid residues, is a multifunctional glycoprotein that induces macrophage differentiation.1 LIF is produced by T cells, fibroblasts, liver, and

Stem Cellutions Newsletter: 2007, Volume 1

: DBA-2N embryonic stem cells passage 67 on mouse embryonic fibroblast cells. Figure 3: Offspring born after injection of FVB/N #17 passage 18 ES cells into C57Bl/6N blastocysts. Left mouse shows 100

Product Information Sheet - SHCLND

SupT1 T-cells ECS mouse embryonic stem cells HCT116 colon carcinoma BJ-TERT diploid fibroblasts fibroblasts mouse embryonic fibroblasts MCF7 breast carcinoma Cell lines, mouse Cell Type

Product Information Sheet - SHCLNG

SupT1 T-cells ECS mouse embryonic stem cells HCT116 colon carcinoma BJ-TERT diploid fibroblasts fibroblasts mouse embryonic fibroblasts MCF7 breast carcinoma Cell lines, mouse Cell Type

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