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关键词:'P7634'
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G P Pfeifer et al.
Science (New York, N.Y.), 246(4931), 810-813 (1989-11-10)
Genomic sequencing permits studies of in vivo DNA methylation and protein-DNA interactions, but its use has been limited because of the complexity of the mammalian genome. A newly developed genomic sequencing procedure in which a ligation mediated polymerase chain reaction
Perturbation of phosphoglycerate kinase 1 (PGK1) only marginally affects glycolysis in cancer cells.
Chengmeng Jin et al.
The Journal of biological chemistry, 295(19), 6425-6446 (2020-03-29)
Phosphoglycerate kinase 1 (PGK1) plays important roles in glycolysis, yet its forward reaction kinetics are unknown, and its role especially in regulating cancer cell glycolysis is unclear. Here, we developed an enzyme assay to measure the kinetic parameters of the
Toshio Ota et al.
Nature genetics, 36(1), 40-45 (2004-01-01)
As a base for human transcriptome and functional genomics, we created the "full-length long Japan" (FLJ) collection of sequenced human cDNAs. We determined the entire sequence of 21,243 selected clones and found that 14,490 cDNAs (10,897 clusters) were unique to
Dong-Song Nie et al.
Molecular biology reports, 40(2), 1905-1910 (2012-10-20)
SPATA17 is a new testis-specific-expressed gene that is involved in Spermatogenesis process. Previous studies show that SPATA17 was involved in acceleration of cell apoptosis in GC-1 cell lines. To further investigate specific roles of SPATA17 in Spermatogenesis in vivo, we
Kayla Baretta et al.
Acta crystallographica. Section F, Structural biology and crystallization communications, 68(Pt 7), 790-792 (2012-07-04)
Acinetobacter baumannii is a common multidrug-resistant clinical pathogen that is often found in hospitals. The A. baumannii phosphoglycerate kinase (AbPGK) is involved in the key energy-producing pathway of glycolysis and presents a potential target for antibiotic development. AbPGK has been
Xinping Chen et al.
Nature chemical biology, 11(1), 19-25 (2014-11-11)
Drugs that can protect against organ damage are urgently needed, especially for diseases such as sepsis and brain stroke. We discovered that terazosin (TZ), a widely marketed α1-adrenergic receptor antagonist, alleviated organ damage and improved survival in rodent models of
Paule Bénit et al.
EBioMedicine, 17, 75-87 (2017-02-24)
Mice with the hypomorphic AIF-Harlequin mutation exhibit a highly heterogeneous mitochondriopathy that mostly affects respiratory chain complex I, causing a cerebral pathology that resembles that found in patients with AIF loss-of-function mutations. Here we describe that the antidiabetic drug pioglitazone
Aurélie Delzor et al.
Human gene therapy methods, 23(4), 242-254 (2012-09-01)
Tissue-targeted expression is of major interest for studying the contribution of cellular subpopulations to neurodegenerative diseases. However, in vivo methods to investigate this issue are limited. Here, we report an analysis of the cell specificity of expression of fluorescent reporter
Stefano Falone et al.
Journal of cellular physiology, 231(9), 2014-2025 (2016-01-13)
Extremely low frequency magnetic fields (ELF-MF) are common environmental agents that are suspected to promote later stages of tumorigenesis, especially in brain-derived malignancies. Even though ELF magnetic fields have been previously linked to increased proliferation in neuroblastoma cells, no previous
A Yoshida et al.
Blood cells, molecules & diseases, 21(3), 179-181 (1995-01-01)
The molecular abnormality of a phosphoglycerate kinase variant associated with severe red cell enzyme deficiency ( about 4% of normal) and episodes of hemolysis with jaundice was examined. The Michaelis constants for the substrates and co-enzymes (1.3-diphosphoglycerate, 3-phosphoglycerate, ATP and
Theresa Kouril et al.
The FEBS journal, 280(18), 4666-4680 (2013-07-20)
Four enzymes of the gluconeogenic pathway in Sulfolobus solfataricus were purified and kinetically characterized. The enzymes were reconstituted in vitro to quantify the contribution of temperature instability of the pathway intermediates to carbon loss from the system. The reconstituted system
Jesper V Olsen et al.
Science signaling, 3(104), ra3-ra3 (2010-01-14)
Eukaryotic cells replicate by a complex series of evolutionarily conserved events that are tightly regulated at defined stages of the cell division cycle. Progression through this cycle involves a large number of dedicated protein complexes and signaling pathways, and deregulation
H Fujii et al.
Blood, 79(6), 1582-1585 (1992-03-15)
We have determined a single amino acid substitution in a new phosphoglycerate kinase (PGK) variant, PGK Shizuoka, associated with chronic hemolysis and myoglobinuria. PGK Shizuoka had an extremely low enzyme activity with normal kinetic properties and normal electrophoretic mobility. Total
Phosphoglycerate kinase: structural aspects and functions, with special emphasis on the enzyme from Kinetoplastea
Pirela MR, et al.
Open Biology (2020)
Wen-Juan Zhang et al.
Oncology reports, 29(2), 491-506 (2012-11-09)
MLAA-34 is a novel acute monocytic leukemia (M5)-associated antigen (MLAA) that plays a role in the apoptosis of U937 cells. However, the expression and molecular mechanism of MLAA-34 in U937 cells remain largely unclear. Here, we utilized three strategies to
Thomas R Burkard et al.
BMC systems biology, 5, 17-17 (2011-01-29)
On the basis of large proteomics datasets measured from seven human cell lines we consider their intersection as an approximation of the human central proteome, which is the set of proteins ubiquitously expressed in all human cells. Composition and properties
J R McCarrey et al.
Nature, 326(6112), 501-505 (1987-04-02)
Phosphoglycerate kinase (PGK) (ATP:3-phospho-D-glycerate 1-phosphotransferase, EC 2.7.2.3) is a metabolic enzyme functioning in the Embden-Meyerhof pathway that converts glucose (or fructose) to pyruvate. Two functional loci for the production of PGK have been identified in the mammalian genome. PGK-1 is
Enzyme analysis. The rationale and use of enzyme assays in assigning function to gene nucleotide sequences and the procedures for the assay of three enzymatic functions conserved in mollicutes.
J D Pollack
Methods in molecular biology (Clifton, N.J.), 104, 79-93 (1998-08-26)
Phosphoglyceryl kinase in higher plants.
B AXELROD et al.
The Journal of biological chemistry, 204(2), 939-948 (1953-10-01)
Angelika B Riemer et al.
Experimental dermatology, 21(8), 625-629 (2012-07-11)
Based on the exquisite sensitivity, reproducibility and wide dynamic range of quantitative reverse-transcription real-time polymerase chain reaction (qRT-PCR), it is currently the gold standard for gene expression studies. Target gene expression is calculated relative to a stably expressed reference gene.
C Valentin et al.
Human mutation, 12(4), 280-287 (1998-09-23)
Phosphoglycerate kinase (PGK) is a X-linked enzyme that plays a key role in the glycolytic pathway. Twelve different variants have already been reported. We describe a new PGK variant, PGK Herlev (Asp 285-->Val), in a 69-year-old Danish patient with isolated
H Fujii et al.
The Journal of biological chemistry, 255(13), 6421-6423 (1980-07-10)
The structural abnormality of the phosphoglycerate kinase variant, PGK München, associated with red cell enzyme deficiency and heat instability, was elucidated by a microscale peptide-mapping method. A single amino acid substitution, from aspartic acid in the normal enzyme to asparagine
Crystalline phosphoglycerate kinase from human erythrocytes.
T HASHIMOTO et al.
Biochimica et biophysica acta, 65, 355-357 (1962-12-04)
Xiaobing Zhu et al.
The Journal of biological chemistry, 296, 100369-100369 (2021-02-06)
Previous studies have identified GAPDH as a promising target for treating cancer and modulating immunity because its inhibition reduces glycolysis in cells (cancer cells and immune cells) with the Warburg effect, a modified form of cellular metabolism found in cancer
Melissa F Jackson et al.
The FEBS journal, 280(3), 827-839 (2012-11-24)
The genetic manipulation of skeletal muscle cells in vitro is notoriously difficult, especially when using undifferentiated muscle cell lines (myoblasts) or primary muscle stem cells (myosatellites). We therefore optimized methods of gene transfer by overexpressing green fluorescent protein (GFP) in
Angel L Pey
Biochimica et biophysica acta, 1834(12), 2502-2511 (2013-08-06)
Conformational diseases often show defective protein folding efficiency in vivo upon mutation, affecting protein properties such as thermodynamic stability and folding/unfolding/misfolding kinetics as well as the interactions of the protein with the protein homeostasis network. Human phosphoglycerate kinase 1 (hPGK1)
H K Jindal et al.
The Journal of biological chemistry, 265(12), 6540-6543 (1990-04-25)
Primer recognition proteins (PRP) are cofactors of DNA polymerase alpha and may have a role in lagging strand DNA replication. Purified PRP from HeLa cells and human placenta are composed of two subunits of 36,000 (PRP 1) and 41,000 (PRP
Qianni Hu et al.
Molecular phylogenetics and evolution, 69(3), 805-813 (2013-07-09)
Increasing evidence has shown the complex and dynamic nature of polyploids. Two single copy nuclear genes were used to explore genome evolutionary dynamics and the origin of tetraploid E. ciliaris: the phosphoglycerate kinase (PGK1) and the second largest subunit of
M Cohen-Solal et al.
Blood, 84(3), 898-903 (1994-08-01)
Phosphoglycerate kinase (PGK) deficiency is generally associated with chronic hemolytic anemia, although it can be accompanied by either mental retardation or muscular disease. Genomic DNAs of two PGK-deficient patients previously described in France were sequenced directly after polymerase chain reaction
Zhangsheng Yang et al.
Reproduction (Cambridge, England), 146(5), 501-516 (2013-09-04)
The phosphoglycerate kinase-2 (Pgk2) gene is regulated in a tissue-, cell type-, and developmental stage-specific manner during spermatogenesis and is required for normal sperm motility and fertility in mammals. Activation of Pgk2 transcription is regulated by testis-specific demethylation of DNA
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