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Moitrayee Bhattacharyya et al.
eLife, 9 (2020-03-10)
The many variants of human Ca2+/calmodulin-dependent protein kinase II (CaMKII) differ in the lengths and sequences of disordered linkers connecting the kinase domains to the oligomeric hubs of the holoenzyme. CaMKII activity depends on the balance between activating and inhibitory
Lluc Farrera-Soler et al.
PloS one, 15(9), e0238089-e0238089 (2020-09-10)
A novel severe acute respiratory syndrome coronavirus (SARS-CoV-2) is the source of a current pandemic (COVID-19) with devastating consequences in public health and economic stability. Using a peptide array to map the antibody response of plasma from healing patients (12)
Ingrid J G Burvenich et al.
mAbs, 8(4), 775-786 (2016-04-01)
IgG has a long half-life through engagement of its Fc region with the neonatal Fc receptor (FcRn). The FcRn binding site on IgG1 has been shown to contain I253 and H310 in the CH2 domain and H435 in the CH3
Douglas B Craig et al.
The protein journal, 32(2), 81-88 (2013-01-18)
Single molecule assays were performed on streptavidin-β-galactosidase using a capillary electrophoresis-based protocol in order to assess the suitability of single molecule β-galactosidase assays for adaptation to the detection of single copies of target DNA. The conjugate was found to have
Structural origins of high-affinity biotin binding to streptavidin.
Weber PC et al
Science, 243(4887), 85-88 (1989)
Characterization and crystallization of core streptavidin.
Pahler A et al
The Journal of Biological Chemistry, 262(29), 13933-13937 (1987)
Thao T Le et al.
Analytica chimica acta, 761, 143-148 (2013-01-15)
This paper describes the measurement of the binding affinities of two bifunctional RNA aptamers to their respective ligands. The aptamers comprise either a theophylline or malachite green binding sequence fused to a streptavidin binding sequence. These bifunctional aptamers are shown
Eduard Urich et al.
Scientific reports, 5, 14104-14104 (2015-09-29)
The blood-brain barrier and the blood-cerebrospinal fluid barrier prevent access of biotherapeutics to their targets in the central nervous system and therefore prohibit the effective treatment of neurological disorders. In an attempt to discover novel brain transport vectors in vivo
Polymer Nanoparticles
Progress in Molecular Biology and Translational Science, 104 (2011)
Yun Shu et al.
Lab on a chip, 13(5), 860-865 (2013-01-10)
Labeling of viruses can be used to reveal viral infection pathways and screen potential anti-viral drugs. Complex procedures, including virus cultivation, purification and labeling are involved in traditional virus labeling methods. And the manipulation of living virus brings risk to
Yu W Chu et al.
Chemical communications (Cambridge, England), 49(24), 2397-2399 (2013-01-19)
Described herein is a unique and inexpensive method that outperforms commercial methods that amplify the streptavidin-biotin recognition event. Amplification induced by streptavidin and biotinylated protein causes the formation of a large detectable polymer. This approach enjoys a 100-fold decrease in
Cell-free protein synthesis and assembly on a biochip.
Heyman Y et al
Nature Nanotechnology, 7(6), 374-378 (2012)
Chiou-Miin Wang et al.
Neurobiology of disease, 39(3), 409-422 (2010-05-25)
Vaccination has become an important therapeutic approach to the treatment of Alzheimer's disease (AD), however, immunization with Abeta amyloid can have unwanted, potentially lethal, side effects. Here we demonstrate an alternative peptide-mimotope vaccine strategy using the SDPM1 peptide. SDPM1 is
Identification of the Heparan Sulfate Binding Sites in the Cellular Prion Protein*
Richard G
The Journal of Biological Chemistry (2002)
Deepti Karandur et al.
eLife, 9 (2020-09-10)
Ca2+/calmodulin-dependent protein kinase II (CaMKII) is an oligomeric enzyme with crucial roles in neuronal signaling and cardiac function. Previously, we showed that activation of CaMKII triggers the exchange of subunits between holoenzymes, potentially increasing the spread of the active state
John G Bruno et al.
Microchemical journal : devoted to the application of microtechniques in all branches of science, 115, 32-38 (2014-04-26)
Fifty-two candidate DNA aptamer sequences were selected for binding to the cardiovascular biomarker B-type or brain natriuretic peptide (BNP). Candidate aptamers were screened to rank their relative affinities against BNP by an aptamer-based ELISA-like aptamer microplate assay (ELASA). The highest
An IVUS transducer for microbubble therapies.
Kilroy JP et al
IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control, 61(3), 441-449 (2014)
Nathaniel G Hentz et al.
Journal of laboratory automation, 19(2), 153-162 (2013-09-14)
This study illustrates how optimization of both liquid-handling accuracy and precision is critical to assay performance. The study was designed to examine (1) liquid-handling performance and (2) the effect of liquid-handling variability on two types of in vitro biochemical assays
Determination of binding curves via protein micropatterning in vitro and in living cells
Stefan S
Cytometry (2012)
Michael Lehnert et al.
Langmuir : the ACS journal of surfaces and colloids, 29(6), 1732-1737 (2013-01-15)
The biomodification of surfaces, especially titanium, is an important issue in current biomedical research. Regarding titanium, it is also important to ensure a specific protein modification of its surface because here protein binding that is too random can be observed.
Javier Sotres et al.
Chemphyschem : a European journal of chemical physics and physical chemistry, 9(4), 590-599 (2008-02-26)
Atomic force microscopy is a technique capable to study biological recognition processes at the single-molecule level. In this work we operate the AFM in a force-scan based mode, the jumping mode, where simultaneous topographic and tip-sample adhesion maps are acquired.
Yoav Gal et al.
Antibodies (Basel, Switzerland), 9(1) (2020-02-12)
Abrin and ricin are potent AB toxins, which are considered biological threats. To date, there are no approved treatments against abrin or ricin intoxications. Previously, we showed that the administration of polyclonal anti-abrin antibodies to mice that were intranasally exposed
Crystal structure of core streptavidin determined from multiwavelength anomalous diffraction of synchrotron radiation.
Hendrickson WA et al
Proceedings of the National Academy of Sciences of the USA, 86(7), 2190-2194 (1989)
Patrick Seelheim et al.
Biochemical and biophysical research communications, 431(3), 519-523 (2013-01-17)
While transmembrane proteins and transporters comprise one of the largest protein families, their use in biosensors like biochips or lab-on-a-chip devices has so far been limited by their demanding requirements of a stable and compartmentalized lipid environment. A possible remedy
The properties of streptavidin, a biotin-binding protein produced by Streptomycetes
Louis C
Archives of Biochemistry and Biophysics, 106 (1964)
THE PROPERTIES OF STREPTAVIDIN, A BIOTIN-BINDING PROTEIN PRODUCED BY STREPTOMYCETES.
L CHAIET et al.
Archives of biochemistry and biophysics, 106, 1-5 (1964-07-20)
Nicola Ilk et al.
Applied and environmental microbiology, 68(7), 3251-3260 (2002-06-29)
The nucleotide sequence encoding the crystalline bacterial cell surface (S-layer) protein SbpA of Bacillus sphaericus CCM 2177 was determined by a PCR-based technique using four overlapping fragments. The entire sbpA sequence indicated one open reading frame of 3,804 bp encoding
Andrew R Harris et al.
Nature communications, 11(1), 5973-5973 (2020-11-27)
The assembly of actin filaments into distinct cytoskeletal structures plays a critical role in cell physiology, but how proteins localize differentially to these structures within a shared cytoplasm remains unclear. Here, we show that the actin-binding domains of accessory proteins
Aaron J Mercer et al.
Current protocols in neuroscience, Chapter 2, Unit 2-Unit 2 (2013-01-15)
At synapses in the central nervous system, precisely localized assemblies of presynaptic proteins, neurotransmitter-filled vesicles, and postsynaptic receptors are required to communicate messages between neurons. Our understanding of synaptic function has been significantly advanced using electrophysiological methods, but the dynamic
Simplified Paper Format for Detecting HIV Drug Resistance in Clinical Specimens by Oligonucleotide Ligation
Nuttada Panpradist
PLoS ONE (2016)
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