Layer by layer assembly of biotinylated protein networks for signal amplification.

Layer by layer assembly of biotinylated protein networks for signal amplification.

Chemical communications (Cambridge, England) (2013-01-19)

Yu W Chu, Bo Y Wang, Huei-Shian Lin, Tai-Yen Lin, Yuan-Jen Hung, David A Engebretson, Wonjae Lee, James R Carey

摘要

Described herein is a unique and inexpensive method that outperforms commercial methods that amplify the streptavidin-biotin recognition event. Amplification induced by streptavidin and biotinylated protein causes the formation of a large detectable polymer. This approach enjoys a 100-fold decrease in detection limit in comparison with the commercial methods.

材料

货号

品牌

产品描述

85878

Sigma-Aldrich

链亲和素来源于阿维丁链霉菌, affinity purified, lyophilized from 10 mM potassium phosphate, ≥13 U/mg protein

S4762

Sigma-Aldrich

链亲和素来源于阿维丁链霉菌, essentially salt-free, lyophilized powder, ≥13 units/mg protein

S0677

Sigma-Aldrich

链亲和素来源于阿维丁链霉菌, recombinant, expressed in E. coli, lyophilized powder

A8706

Sigma-Aldrich

亲和素来源于鸡蛋白, recombinant, expressed in corn, ≥12 units/mg protein (E1%/280)