The upstream workflow encompasses all steps leading to cell harvest including optimization of culture conditions, cell line and product characterization, media development, upstream process chemicals; and scale-up of the batch, fed-batch or perfusion process to larger bioreactors. The goal of upstream biomanufacturing is to increase cell density and product titer.
Bioreactors
Bioreactors are available in a range of sizes and formats (stainless steel, single-use, or hybrid) to support both adherent and suspension cell cultures. Adherent cell lines can be grown in batch or fed-batch modes, or with media exchange, while suspension cells can be grown in batch, fed-batch, and perfusion modes. Regardless of the cell type or operating mode, the bioreactor must enable consistent cell growth, high cell viability, and robust protein production. During process development, scaled-down, small working volume microbioreactors are used to predict the performance of larger scale vessels, facilitating the process of scale-up for clinical and commercial production or tech transfer.
Our comprehensive offering around the bioreactor includes:
- Millipore® Single-Use Bioreactors With or Without Cell Retention System for Perfusion Operations
- Millipore® Sterilizing Filters & Prefilters
- Millipore® Sampling Solutions (automated online system, offline sampling)
- Millipore® Process Analytical Technology (Raman Analyzer, automated aseptic sampling)
- Millipore® CTDMO, BioReliance® / Millipore® Services (cell and viral bank testing, raw material testing, bulk harvest release testing / single-use and filter validation, equipment installation, system qualification, system training, system services)
- Millipore® Automation and Analytics Software
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A cell line that maintains favorable growth and productivity is vital for mab manufacturing.
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Our recombinant, non-animal origin (NAO) supplements are formulated for cell culture applications.
Mobius® single-use mixing solutions include a complete range of mixers from 10 L to 3,000 L.
The Mobius® Bioreactor family accommodates batch, fed-batch, and perfusion upstream processes.
Our broad portfolio of membrane filters offers options for efficient processing of every pharma and biopharma stream.
Related Resources
- Upstream Bioprocessing Feed Strategies and Scalability: A Case Study with Cellvento® ModiFeed Prime COMP
Prior to scaling your upstream bioprocess to bioreactors, it’s best to set up small-scale cultures to determine the media and feed combinations as well as other parameters to best optimize growth, productivity, and product quality from your cells.
- In-Line Real-Time Monitoring of CHO Cell Culture Process Parameters using Raman Spectroscopy
This article explains how ProCellics™ Raman Analyzer with Bio4C® PAT Raman Software (the Raman PAT Platform) can be used to perform in-line and real-time measurement of TCD, VCD and mAb titer as well as the concentration of glucose, lactate, and ammonium in a bench-scale bioreactor, and how it compares with off line monitoring.
- Brochure: Mission Control: Strategies for Effective Bioburden and Aseptic Control
In this series of articles, we provide information to highlight the differing objectives for bioburden control by unit operation and discuss the parameters that guide filter selection and optimization.
- Cell Culture Media Filtration, Filter Selection and Sizing
The purpose of this application note is to provide estimated filtration areas for different sterilizing-grade filters with a panel of media used for Chinese Hamster Ovary (CHO) cell culture processes.
- Cell Culture Media Mixing and Microfiltration: Reducing Risk with Single-use Technology
This case study presents our recommended methods for thoroughly mixing media, as well as reducing the risk of mycoplasma contamination through the use of 0.1 μm sterilizing-grade filters.
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