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W4502SIGMA

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关键词:'W4502SIGMA'
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Imprint®甲基化DNA定量试剂盒(MDQ1)实验方案
Imprint®甲基化DNA定量试剂盒提供了一种高通量、非放射性的整体DNA甲基化变化测定方法。
完整全转录组扩增试剂盒实验方案(WTA2)
WTA2(一种全转录组扩增(WTA)方法)能够在区区4小时内,代表性地扩增出纳克级的总RNA而没有3'偏差。
GenElute™ 植物基因组DNA Miniprep试剂盒实验方案(G2N10, G2N70, G2N350)
本方案描述了使用GenElute植物基因组DNA Miniprep试剂盒,从多种植物中分离纯DNA的简单、便捷操作步骤。
逆转录实验方案
将RNA逆转录成DNA的方法。使用含有逆转录酶、dNTP、引物、RNase抑制剂和缓冲液的预混试剂。快速合成cDNA。
SEQR – SeqPlex RNA扩增试剂盒实验方案
SeqPlex RNA扩增试剂盒可在进入常用深度测序平台工作流程之前为总RNA或分离后的mRNA提供一种扩增方法,
引物浓度优化实验方案
引物浓度优化实验方案是一种建立反应阵列的方法。该阵列用来对照伴侣引物的不同浓度来测试每种引物的一系列浓度。
使用SYBR Green染料检测的逆转录实验方案
使用逆转录酶和dNTPs进行逆转录(RT)使用总RNA或基因特异性方法实现仅目标RNA被转化为cDNA。
标准逆转录方案(两步法)
逆转录是一种旨在测量基因mrna浓度的基因表达分析。 这种分析存在多个挑战,例如不同转录本之间的半衰期差异、时间模式和缺乏相关蛋白。
GenElute™ 哺乳动物基因组 DNA 微量提取试剂盒实验方案
GenElute 哺乳动物基因组 DNA 纯化方案阐述了从多种哺乳动物来源中分离纯高分子量 DNA 的方法。
选择qPCR参照基因的实验方案
基因表达数据的分析需要稳定的参考或上样控制。所述参考通常是一个或多个参照基因。
GenElute™细菌基因组DNA试剂盒实验方案(NA2100、NA2110、NA2120)
的GenElute™细菌基因组DNA试剂盒提供了一种从细菌中分离纯基因组DNA的简便方法。
使用温度梯度的引物优化试验方案
用于测定优化的梯度PCR通过在一系列退火温度下测试含有固定引物浓度的相同反应物来确定引物的最佳退火温度(Ta)。
qPCR效率测定实验方案
QPCR 条件的优化对于制定稳健的检测方案非常重要。两种主要优化方法是优化引物浓度和/或退火温度。
使用SYBR Green I染料检测的qPCR基因表达/拷贝数分析实验方案
使用 SYBR Green I 染料检测一个或多个稳定内参基因的靶量是qPCR的常见应用。下面是一个可以适应特定实验需要的标准实验方案。
利用 CRISPR-Cas9 基因组编辑技术创建转基因小鼠
使用CRISPR/Cas9系统生成正确的转基因小鼠需要充分理解正在测试的假设。了解如何创建成功的小鼠模型项目。
Imprint® Methylated DNA Quantification Kit (MDQ1) Protocol
Imprint ® Methylated DNA Quantification Kit (MDQ1) Protocol
GenomePlex® Single Cell Whole Genome Amplification Kit (WGA4) - Protocol
GenomePlex® is a Whole Genome Amplification (WGA) method that allows the researcher to generate a representative amplification of genomic DNA
Complete Whole Transcriptome Amplification Kit Protocol (WTA2)
WTA2, a Whole Transcriptome Amplification (WTA) method, allows for representative amplification of nanogram quantities of total RNA in less than 4 hours without 3-bias
Whole Genome Amplification from Serum or Plasma Protocol
Whole genome amplification (WGA) of plasma and serum DNA presents a unique challenge due to the small amount of nucleic acid in such samples.
GenElute™ Plant Genomic DNA Miniprep Kit Protocol (G2N10, G2N70, G2N350)
This protocol describes a simple and convenient procedure to isolate pure DNA from a variety of plant species using the GenElute Plant Genomic DNA Miniprep Kit.
Preparation of Antibody Sensitized Sheep Erythrocytes
Protocol for the preparation of Antibody Sensitized Sheep Erythrocytes. Includes product numbers and links.
Saliva DNA Extraction & WGA Amplification
Whole Genome Amplification products, including kits for DNA extraction, support various DNA sources.
Animal Tissue DNA-Extraction & WGA Amplification Protocol
GenomePlex® Whole Genome Amplification efficiently extracts DNA from animal samples for genomic analysis.
Reverse Transcription Protocol
Method for reverse transcription of RNA into DNA. Uses a premixed reagent that contains reverse transcriptase, dNTPs, primers, RNase inhibitor and buffer. Fast generation of cDNA.
SEQR - SeqPlex RNA Amplification Kit Protocol
The SeqPlex RNA Amplification kit provides a method for amplification of total RNA or isolated mRNA prior to entry into the workflows of the commonly used deep sequencing platforms.
Blood Card - WGA Protocol
Protocol extracts genomic DNA from blood cards, useful for limited DNA samples in amplification.
Extraction & Amplification of Whole Blood Using WGA-Protocol
Simple DNA isolation protocol from whole blood samples for genomic analysis, followed by GenomePlex® amplification.
Reverse Transcription Protocol (One-step Probe Detection)
Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.
Buccal DNA Extraction & WGA Amplification Protocol
This protocol provides a simple and convenient method to isolate, amplify, and purify genomic DNA from buccal swabs. Buccal swabs are a convenient method of acquiring a DNA sample.
Plant DNA Extraction & WGA Amplification Protocol
GenomePlex® Whole Genome Amplification has been used to amplify genomic DNA from soybean, corn, tomato, purple coneflower, and ginseng.
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