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AB3203

Sigma-Aldrich

Anti-Tubulin Antibody, δ 2

Chemicon®, from rabbit

Synonym(s):

Anti-α-tubulin, Tubulin antibody

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50 μG
CN¥6,550.17

CN¥6,550.17


Estimated to ship onAugust 11, 2025Details

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50 μG
CN¥6,550.17

About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

CN¥6,550.17


Estimated to ship onAugust 11, 2025Details

Promotion available. Please contact an authorized dealer to order.

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biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

sea urchin, echinoderm, plant

should not react with

Saccharomyces cerevisiae

species reactivity (predicted by homology)

mammals

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T9822T3195ABT171
antibody form

affinity purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

IgG fraction of antiserum

antibody form

serum

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

100

biological source

rabbit

biological source

mouse

biological source

rabbit

biological source

rabbit

UniProt accession no.

Q9UJT1

UniProt accession no.

P68363

UniProt accession no.

P23258

UniProt accession no.

Q71U36

clone

polyclonal

clone

B3, monoclonal

clone

polyclonal

clone

polyclonal

shipped in

wet ice

shipped in

dry ice

shipped in

dry ice

shipped in

wet ice

General description

Tubulin, the dimeric subunit of microtubules, is submitted to post translational modifications including cyclic tyrosine removal and addition at the COOH terminus of the alpha subunit. The cycle involves two enzymes, TTL (Tubuline Tyrosine Ligase) and Tubulin carboxypeptidase, and generates two major forms: tyrosinated tubulin and detyrosinated tubulin (Glu-tubulin). Removal of the COOH-terminal Glu residue from the alpha chain of Glu-tubulin generates the delta2-tubulin form.

Specificity

The antibody specifically recognizes the delta 2 form of the tubulin alpha chain (Paturle-Lafanechere et al. 1991).

SPECIES REACTIVITY:

Mammals, sea urchin, plants, not yeast (Lafanechere et al. 1994).

Immunogen

Peptide corresponding to the C-terminal of delta 2 tubulin.

Application

Research Category
Cell Structure
Research Sub Category
Cytoskeleton
This Anti-Tubulin Antibody, δ 2 is validated for use in IH(P), WB, IC, IH for the detection of Tubulin.
Western blot: 1:1,000

Immunohistochemistry and EM on either frozen or paraffin embedded, fixed tissue (Mialhe et al. 2001): 1:500

Optimal working dilutions must be determined by the end user.

Physical form

Affinity purified immunoglobulin. Liquid in PBS.

Preparation Note

Maintain at 2-8°C in undiluted for up to 6 months from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


  • Certificates of Analysis (COA)

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    Hung-Chi Liu et al.
    Plant physiology, 191(1), 177-198 (2022-10-23)
    Phosphoglucose isomerase (PGI) catalyzes the interconversion of fructose-6-phosphate and glucose-6-phosphate, which impacts cell carbon metabolic flow. Arabidopsis (Arabidopsis thaliana) contains two nuclear PGI genes respectively encoding plastidial PGI1 and cytosolic PGI (cPGI). The loss of PGI1 impairs the conversion of
    Robert O'Hagan et al.
    Current biology : CB, 21(20), 1685-1694 (2011-10-11)
    Posttranslational modifications (PTMs) such as acetylation, detyrosination, and polyglutamylation have long been considered markers of stable microtubules and have recently been proposed to guide molecular motors to specific subcellular destinations. Microtubules can be deglutamylated by the cytosolic carboxypeptidase CCP1. Loss
    Post-translational modifications to Toxoplasma gondii alpha- and beta-tubulins include novel C-terminal methylation.
    Xiao, H; El Bissati, K; Verdier-Pinard, P; Burd, B; Zhang, H; Kim, K; Fiser, A; Angeletti et al.
    Journal of Proteome Research null
    Kinesin-13 and tubulin posttranslational modifications regulate microtubule growth in axon regeneration.
    Ghosh-Roy, A; Goncharov, A; Jin, Y; Chisholm, AD
    Developmental Cell null
    Dynamics of DNA methylation and Histone H4 acetylation during floral bud differentiation in azalea.
    Meijon, M; Feito, I; Valledor, L; Rodriguez, R; Ca?al, MJ
    BMC plant biology null

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