Skip to Content
Merck
CN

Key Documents

View All Documentation

10393343001

Roche

T7 RNA Polymerase, rec.

pkg of 30 kU, (AOF, Antibiotic Free Production, Extended Impurity tested)

Synonym(s):

bacteriophage T7 RNA polymerase, T7 RNAP, e coli T7 RNA polymerase, in vitro mRNA polymerase, recombinant T7

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

UNSPSC Code:
12352204
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

form

solution

packaging

pkg of 30 kU

manufacturer/tradename

Roche 10393343001

storage temp.

−20°C

General description

T7 RNA Polymerase, rec. is the key enzyme for in vitro transcription of mRNA. It generates mRNA from double-stranded DNA templates carrying the T7 promoter sequence and efficiently incorporates standard or modified ribonucleoside triphosphates (NTPs) into mRNA transcripts.Its high specificity and processivity enable the production of large quantities of RNA with minimal by-products, facilitating downstream applications.

Application

Synthesized RNA can be used for RNA/DNA blotting techniques, in situ hybridization, RNase protection studies, synthesis of capped RNA, and microarray target synthesis.

Features and Benefits

  • High Efficiency: T7 RNA Polymerase exhibits a high transcription rate, enabling the rapid synthesis of large quantities of mRNA from DNA templates. This efficiency is beneficial for experiments requiring substantial amounts of RNA.


  • Specificity for T7 Promoter: The enzyme specifically recognizes and binds to the T7 promoter sequence, ensuring accurate transcription initiation and reducing the likelihood of non-specific products.


  • Versatile NTP Incorporation: T7 RNA Polymerase can incorporate a range of standard and modified ribonucleoside triphosphates, allowing researchers to tailor RNA transcripts for specific applications and studies.


  • Minimal By-products: The enzyme′s high fidelity and processivity result in the generation of clean RNA products with minimal by-products, enhancing the quality of the synthesized transcripts.


  • Scalable Production: The enzyme′s robust nature allows for scalable production of mRNA, making it suitable for both small-scale laboratory experiments and large-scale applications in biotechnology and pharmaceuticals.

Still not finding the right product?

Explore all of our products under T7 RNA Polymerase, rec.

Regulatory Information

新产品

This item has

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library