YTH 906.9HL
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., 11060809
biological source
Rat x Rat Hybridoma
packaging
tube of 5 μg 11060809-DNA-5UG
pkg of vial of cells 11060809-1VL
ImmunoDon
DA rat spleen
Myeloma
Y3.AG.1.2.3
Reactivity
Human IL2 receptor alpha (CD25)
technique(s)
cell culture | mammalian: suitable
isotype
IgG2b
shipped in
dry ice
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Immunogen
Activated human T cells
Biochem/physiol Actions
Monoclonal antibody, mouse CD4 epitope A
Rat hybridoma producing antibody against human CD25. The designation HL indicates that it has bselected for loss of the original Y3 myeloma derived (non-specific) light chain. Cambridge Enterprise Reference number AB61:www.enterprise.cam.ac.uk.
Preparation Note
IDMEM (I3390) + 2 mM L-Glutamine (G7513) + 5% FBS / FCS (F2442). Where the cells are grown under static conditions, 0.02% EDTA, Trypsin (T4424) or mechanical agitation maybe required to dislodge them from the plastic.
Maintain cultures between 3-9 x 105 cells/ml. 5% CO2; 37 °C. Saturation density 9 x 105/ml.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
Regulatory Information
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