CARM-L1 TG3
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
Synonym(s):
CARM, TG3
biological source
rat lung
packaging
tube of 5 μg 11120835-DNA-5UG
pkg of vial of cells 11120835-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial and spindle shaped biphasic morphology
receptors
Not specified
relevant disease(s)
metastasis
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Biochem/physiol Actions
Not specified
Rat transplantable mesothelioma asbestos, primary tumour
The Me-series of esotheliomas were generated by an intrapleural inoculation of UICC crocidolite to induce primary mesotheliomas in syngenic PVG/c Norweigan Hooded rats. Solid fragments of the tumours were transplanted and maintained by s.c. serial passage. Reference cultures designated CARM-lines (CARM-L12 TG3 11120833 and CARM-L1 TG3 11120835) were derived from Me9-TG3 (Sigma product number 11120841) by cloning the cells three times in soft agar. Each line was selected on the basis of their epithelial or fibroblastic phenotype. The cell lines are metastatic in a syngeneic host.
Preparation Note
Split subconfluent cultures (70-80%) 1:4 to 1:10 using 0.25% trypsin/EDTA; 5% CO2; 37 °C. Suggested seeding density 2-4 x 10,000 cells/cm2.
Other Notes
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Regulatory Information
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