CARM-L1 TG3

Not specified

Rat transplantable mesothelioma asbestos, primary tumour

The Me-series of esotheliomas were generated by an intrapleural inoculation of UICC crocidolite to induce primary mesotheliomas in syngenic PVG/c Norweigan Hooded rats. Solid fragments of the tumours were transplanted and maintained by s.c. serial passage. Reference cultures designated CARM-lines (CARM-L12 TG3 11120833 and CARM-L1 TG3 11120835) were derived from Me9-TG3 (Sigma product number 11120841) by cloning the cells three times in soft agar. Each line was selected on the basis of their epithelial or fibroblastic phenotype. The cell lines are metastatic in a syngeneic host.

DMEM (D6546)+ L-Glutamine (G7513) + 10% FBS / FCS (F2442).

Split subconfluent cultures (70-80%) 1:4 to 1:10 using 0.25% trypsin/EDTA; 5% CO₂; 37 °C. Suggested seeding density 2-4 x 10,000 cells/cm².

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.