form
prepacked column
crosslinking
cross-linked
mol wt
protein Mr 5000 Da (exclusion limit)
packaging
pkg of 1 ea
manufacturer/tradename
Cytiva 17-5087-01
storage condition
(20% ethanol)
parameter
≤15 mL sample volume, 40 mL/min max. flow rate, 5 bar max. pressure, 9-31 mL/min flow rate
technique(s)
liquid chromatography (LC): suitable
bed H × diam.
10 cm × 2.6 cm
bed volume
53 mL
matrix
cross-linked dextran
matrix active group
dextran-epichlorohydrin phase, dextran
particle size
20-80 μm (dry)
cleaning
2-13
working range
2-13
separation technique
size exclusion (SEC)
shipped in
ambient
storage temp.
2-30°C
General description
HiPrep™ 26/10 Desalting is a convenient, easy-to-use column prepacked 53 mL column with Sephadex® G-25 Fine for desalting and buffer exchange.
Application
All commonly used buffers, 0.2 M NaOH, 0.2 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 24 % ethanol, 30 % porpanol, 30 % acetonitrile
for buffer exchange prior to or after different chromatographic steps.
Features and Benefits
- Provide reliable and reproducible desalting and buffer exchange with sample sizes of up to 15 mL per column.
- Replaces dialysis for desalting and therefore saves time and reduces labor.
- Compatible with a laboratory pump or chromatography system such as AKTA design.
- Two or four columns can be used in series for sample sizes of 30 to 60 mL.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Store at 4 to 30 °C (4 to 8, Used Media 20% Ehtanol or 0.01 M NaOH)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
HiPrep is a trademark of Cytiva
Sephadex is a registered trademark of Cytiva
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signalword
Warning
hcodes
存储类别
3 - Flammable liquids
flash_point_f
Not applicable
flash_point_c
Not applicable